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首页> 外文期刊>Journal of bacteriology >Similar and Divergent Effects of ppGpp and DksA Deficiencies on Transcription in Escherichia coli
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Similar and Divergent Effects of ppGpp and DksA Deficiencies on Transcription in Escherichia coli

机译：ppGpp和DksA缺陷对大肠杆菌转录的相似和不同作用

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The concerted action of ppGpp and DksA in transcription has been widely documented. In disparity with this model, phenotypic studies showed that ppGpp and DksA might also have independent and opposing roles in gene expression in Escherichia coli. In this study we used a transcriptomic approach to compare the global transcriptional patterns of gene expression in strains deficient in ppGpp (ppGpp0) and/or DksA (ΔdksA). Approximately 6 and 7% of all genes were significantly affected by more than twofold in ppGpp- and DksA-deficient strains, respectively, increasing to 13% of all genes in the ppGpp0 ΔdksA strain. Although the data indicate that most of the affected genes were copositively or conegatively regulated by ppGpp and DksA, some genes that were independently and/or differentially regulated by the two factors were found. The large functional group of chemotaxis and flagellum synthesis genes were notably differentially affected, with all genes being upregulated in the DksA-deficient strain but 60% of them being downregulated in the ppGpp-deficient strain. Revealingly, mutations in the antipausing Gre factors suppress the upregulation observed in the DksA-deficient strain, emphasizing the importance of the secondary channel of the RNA polymerase for regulation and fine-tuning of gene expression in E. coli.
机译：ppGpp和DksA在转录中的协同作用已被广泛记录。与该模型不同的是，表型研究表明ppGpp和DksA在大肠杆菌中的基因表达中也可能具有独立和相反的作用。在这项研究中，我们使用了转录组学方法，比较了ppGpp（ppGpp 0 ）和/或DksA（Δ dksA ）缺陷的菌株中基因表达的整体转录模式。在ppGpp和DksA缺陷菌株中，所有基因的大约6％和7％受显着影响是两倍多，在ppGpp 0 Δ dksA < / em>应变。尽管数据表明大多数受影响的基因受到ppGpp和DksA的正调控或负调控，但发现一些受两个因素独立和/或差异调控的基因。趋化性和鞭毛合成基因的大功能群受到显着不同的影响，在DksA缺陷型菌株中所有基因均被上调，而在ppGpp缺陷型菌株中所有基因均被下调。令人惊讶的是，抗暂停性Gre因子的突变抑制了在DksA缺陷型菌株中观察到的上调，强调了RNA聚合酶的二级通道对于调控em中E基因表达的重要性。大肠杆菌。

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