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首页> 外文期刊>Journal of bacteriology >Staphylococcus aureus MazF Specifically Cleaves a Pentad Sequence, UACAU, Which Is Unusually Abundant in the mRNA for Pathogenic Adhesive Factor SraP
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Staphylococcus aureus MazF Specifically Cleaves a Pentad Sequence, UACAU, Which Is Unusually Abundant in the mRNA for Pathogenic Adhesive Factor SraP

机译：金黄色葡萄球菌MazF特异切割了五元序列UACAU，该序列在致病性粘附因子SraP的mRNA中异常丰富

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摘要

Escherichia coli mRNA interferases, such as MazF and ChpBK, are sequence-specific endoribonucleases encoded by toxin-antitoxin (TA) systems present in its genome. A MazF homologue in Staphylococcus aureus (MazFSa) has been shown to inhibit cell growth when induced in E. coli. Here, we determined the cleavage site for MazFSa with the use of phage MS2 RNA as a substrate and CspA, an RNA chaperone, which prevents the formation of secondary structures in the RNA substrate. MazFSa specifically cleaves the RNA at a pentad sequence, U↓ACAU. Bioinformatics analysis revealed that this pentad sequence is significantly abundant in several genes, including the sraP gene in the S. aureus N315 strain. This gene encodes a serine-rich protein, which is known to play an important role in adhesion of the pathogen to human tissues and thus in endovascular infection. We demonstrated that the sraP mRNA became extremely unstable in comparison with the ompA mRNA only when MazFSa was induced in E. coli. Further bioinformatics analysis indicated that the pentad sequence is also significantly abundant in the mRNAs for all the pathogenic factors in S. aureus. This observation suggests a possible regulatory relationship between the MazEFSa TA module and the pathogenicity in S. aureus.
机译：大肠杆菌mRNA干扰酶，例如MazF和ChpBK，是由其基因组中的毒素-抗毒素（TA）系统编码的序列特异性核糖核酸内切酶。已证明在 E中诱导的金黄色葡萄球菌中的MazF同源物（MazF Sa ）抑制细胞生长。大肠杆菌。在这里，我们使用噬菌体MS2 RNA作为底物和CspA（一种RNA分子伴侣）来确定MazF Sa 的切割位点，从而阻止了RNA底物中二级结构的形成。 MazF Sa 特异性地以五单元序列U↓ACAU切割RNA。生物信息学分析表明，五联体序列在几个基因中非常丰富，包括在 S中的 sraP 基因。金黄色葡萄球菌N315菌株。该基因编码富含丝氨酸的蛋白质，已知该蛋白质在病原体与人体组织的粘附以及因此在血管内感染中起重要作用。我们证明，只有在 E中诱导了MazF Sa 的情况下，与 ompA mRNA相比， sraP mRNA变得极其不稳定。大肠杆菌。进一步的生物信息学分析表明， S中所有致病因子的五联体序列在mRNA中也显着丰富。金黄色。该观察结果表明MazEF Sa TA模块与 S的致病性之间可能存在调节关系。金黄色。

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