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首页> 外文期刊>Journal of bacteriology >Polycyclic Aromatic Hydrocarbon Metabolic Network in Mycobacterium vanbaaleniiPYR-1
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Polycyclic Aromatic Hydrocarbon Metabolic Network in Mycobacterium vanbaaleniiPYR-1

机译：vanbaaleniiPYR-1分枝杆菌中的多环芳烃代谢网络

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This study investigated a metabolic network (MN) from Mycobacterium vanbaaleniiPYR-1 for polycyclic aromatic hydrocarbons (PAHs) from the perspective of structure, behavior, and evolution, in which multilayer omics data are integrated. Initially, we utilized a high-throughput proteomic analysis to assess the protein expression response of M. vanbaaleniiPYR-1 to seven different aromatic compounds. A total of 3,431 proteins (57.38% of the genome-predicted proteins) were identified, which included 160 proteins that seemed to be involved in the degradation of aromatic hydrocarbons. Based on the proteomic data and the previous metabolic, biochemical, physiological, and genomic information, we reconstructed an experiment-based system-level PAH-MN. The structure of PAH-MN, with 183 metabolic compounds and 224 chemical reactions, has a typical scale-free nature. The behavior and evolution of the PAH-MN reveals a hierarchical modularity with funnel effects in structure/function and intimate association with evolutionary modules of the functional modules, which are the ring cleavage process (RCP), side chain process (SCP), and central aromatic process (CAP). The 189 commonly upregulated proteins in all aromatic hydrocarbon treatments provide insights into the global adaptation to facilitate the PAH metabolism. Taken together, the findings of our study provide the hierarchical viewpoint from genes/proteins/metabolites to the network via functional modules of the PAH-MN equipped with the engineering-driven approaches of modularization and rationalization, which may expand our understanding of the metabolic potential of M. vanbaaleniiPYR-1 for bioremediation applications.
机译：这项研究从结构，行为和演化的角度研究了VanbaaleniiPYR-1的多环芳烃（PAH）的代谢网络（MN），该网络集成了多层组学数据。最初，我们利用高通量蛋白质组学分析来评估M. vanbaaleniiPYR-1对7种不同芳族化合物的蛋白质表达反应。总共鉴定出3,431种蛋白质（占基因组预测蛋白质的57.38％），其中包括160种似乎与芳香烃降解有关的蛋白质。基于蛋白质组学数据和先前的代谢，生化，生理和基因组信息，我们重建了基于实验的系统级PAH-MN。 PAH-MN的结构具有183种代谢化合物和224种化学反应，具有典型的无垢性质。 PAH-MN的行为和演化揭示出具有结构/功能中的漏斗效应的分级模块化，并且与功能模块的演化模块（环裂解过程（RCP），侧链过程（SCP）和中心）紧密相关芳香过程（CAP）。所有芳香烃处理中的189种普遍上调的蛋白质为深入了解促进PAH代谢的全球适应提供了见识。综上所述，我们的研究结果提供了从PAH-MN的功能模块到基因/蛋白质/代谢物到网络的分层观点，这些功能模块配备了工程驱动的模块化和合理化方法，这可能会扩展我们对代谢潜力的理解vanbaaleniiPYR-1用于生物修复应用。

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