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首页> 外文期刊>Journal of bacteriology >Proximity of the Start Codon to a Leaderless mRNA's 5′ Terminus Is a Strong Positive Determinant of Ribosome Binding and Expression in Escherichia coli
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Proximity of the Start Codon to a Leaderless mRNA's 5′ Terminus Is a Strong Positive Determinant of Ribosome Binding and Expression in Escherichia coli

机译:起始密码子对无领导mRNA的5'末端的接近是核糖体结合和在大肠杆菌中表达的强阳性决定因素。

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摘要

An AUG start codon is an important determinant of ribosome binding and expression of leaderless mRNAs in Escherichia coli. Using reporter constructs encoding mRNAs where the AUG start codon is preceded by untranslated leaders of various length and sequence, we find that close proximity of the start codon to the 5′ terminus and the leader sequence are strong determinants of both ribosome binding and expression.
机译:AUG起始密码子是大肠杆菌中核糖体结合和无前导mRNA表达的重要决定因素。使用编码mRNA的报告基因构建体,其中AUG起始密码子之前是各种长度和序列的未翻译前导序列,我们发现起始密码子与5'末端和前导序列的紧密接近是核糖体结合和表达的强决定因素。

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