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首页> 外文期刊>Journal of bacteriology >Effects of Cortex Peptidoglycan Structure and Cortex Hydrolysis on the Kinetics of Ca2+-Dipicolinic Acid Release during Bacillus subtilis Spore Germination
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Effects of Cortex Peptidoglycan Structure and Cortex Hydrolysis on the Kinetics of Ca2+-Dipicolinic Acid Release during Bacillus subtilis Spore Germination

机译：皮质肽聚糖结构和皮质水解对枯草芽孢杆菌孢子萌发过程中Ca2 +-二吡啶甲酸释放动力学的影响

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摘要

The kinetic parameters of the release of Ca2+-dipicolinic acid (CaDPA) during germination of spore populations and multiple individual spores of Bacillus subtilis strains with major alterations in the structure of the spore peptidoglycan (PG) cortex or lacking one or both of the two redundant enzymes involved in cortex hydrolysis (cortex-lytic enzymes [CLEs]) were determined. The lack of the CLE CwlJ greatly slowed CaDPA release with a germinant receptor (GR)-dependent germinant, l-valine, or a non-GR-dependent germinant, dodecylamine. The absence of the cortex-specific PG modification muramic acid–δ-lactam also increased the time needed for full CaDPA release during germination with both types of germinants. In contrast, increased cortex PG cross-linking was associated with faster times for initiation of CaDPA release with both l-valine and dodecylamine but not with faster CaDPA release once this release had been initiated. These data suggest that the precise structure of the spore cortex plays a significant role in determining the timing and the rate of CaDPA release during B. subtilis spore germination and, further, that this effect is independent of effects of GRs.
机译：枯草芽孢杆菌菌株的孢子种群和多个单个孢子萌发过程中，孢子肽聚糖（PG）皮层结构发生重大变化时，Ca 2 + -dipicolinic acid（CaDPA）释放的动力学参数确定是否缺少皮质水解中涉及的两种冗余酶（皮质溶解酶[CLE]）之一或两者都不存在。 CLE CwlJ的缺乏大大降低了CaDPA的释放，其中依赖于发芽受体（GR）的发芽剂l-缬氨酸或不依赖GR的发芽剂十二烷基胺。缺少皮质特异的PG修饰的山mura酸-δ-内酰胺也增加了两种发芽剂在发芽过程中完全释放CaDPA所需的时间。相比之下，皮质PG交联的增加与通过L-缬氨酸和十二烷基胺启动CaDPA释放的更快时间相关，但是与启动该释放后更快的CaDPA释放无关。这些数据表明，孢子皮层的精确结构在确定枯草芽孢杆菌孢子萌发过程中CaDPA释放的时间和速率中起着重要作用，此外，这种作用与GRs的作用无关。

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