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首页> 外文期刊>Journal of bacteriology >Genes Required for Bacillus anthracis Secondary Cell Wall Polysaccharide Synthesis
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Genes Required for Bacillus anthracis Secondary Cell Wall Polysaccharide Synthesis

机译：炭疽芽孢杆菌次级细胞壁多糖合成所需的基因

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The secondary cell wall polysaccharide (SCWP) is thought to be essential for vegetative growth and surface (S)-layer assembly in Bacillus anthracis; however, the genetic determinants for the assembly of its trisaccharide repeat structure are not known. Here, we report that WpaA (BAS0847) and WpaB (BAS5274) share features with membrane proteins involved in the assembly of O-antigen lipopolysaccharide in Gram-negative bacteria and propose that WpaA and WpaB contribute to the assembly of the SCWP in B. anthracis. Vegetative forms of the B. anthracis wpaA mutant displayed increased lengths of cell chains, a cell separation defect that was attributed to mislocalization of the S-layer-associated murein hydrolases BslO, BslS, and BslT. The wpaB mutant was defective in vegetative replication during early logarithmic growth and formed smaller colonies. Deletion of both genes, wpaA and wpaB, did not yield viable bacilli, and when depleted of both wpaA and wpaB, B. anthracis could not maintain cell shape, support vegetative growth, or assemble SCWP. We propose that WpaA and WpaB fulfill overlapping glycosyltransferase functions of either polymerizing repeat units or transferring SCWP polymers to linkage units prior to LCP-mediated anchoring of the polysaccharide to peptidoglycan.
机译：人们认为，次生细胞壁多糖（SCWP）对于炭疽芽胞杆菌的营养生长和表面（S）层组装至关重要。但是，尚不清楚其三糖重复结构的组装的遗传决定因素。在这里，我们报告WpaA（BAS0847）和WpaB（BAS5274）与参与革兰氏阴性细菌O抗原脂多糖组装的膜蛋白共享功能，并提出WpaA和WpaB有助于炭疽芽孢杆菌中SCWP的组装。 。炭疽芽孢杆菌wpaA突变体的营养形式显示出增加的细胞链长度，这是由于与S层相关的壁蛋白水解酶BslO，BslS和BslT错位导致的细胞分离缺陷。在早期对数生长过程中， wpaB 突变体在营养复制方面存在缺陷，并形成了较小的菌落。删除 wpaA 和 wpaB 这两个基因均不产生活菌，而同时删除 wpaA 和 wpaB ，炭疽芽孢杆菌不能维持细胞形状，支持营养生长或组装SCWP。我们建议WpaA和WpaB在聚合LCP介导的多糖锚定到肽聚糖之前，实现聚合重复单元或将SCWP聚合物转移到连接单元的重叠糖基转移酶功能。

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