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首页> 外文期刊>Scientific reports. >Developing genome-wide microsatellite markers of bamboo and their applications on molecular marker assisted taxonomy for accessions in the genus Phyllostachys
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Developing genome-wide microsatellite markers of bamboo and their applications on molecular marker assisted taxonomy for accessions in the genus Phyllostachys

机译：竹的全基因组微卫星标记的开发及其在分子标记辅助分类学中的应用

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Morphology-based taxonomy via exiguously reproductive organ has severely limitation on bamboo taxonomy, mainly owing to infrequent and unpredictable flowering events of bamboo. Here, we present the first genome-wide analysis and application of microsatellites based on the genome of moso bamboo ( Phyllostachys edulis ) to assist bamboo taxonomy. Of identified 127,593 microsatellite repeat-motifs, the primers of 1,451 microsatellites were designed and 1,098 markers were physically mapped on the genome of moso bamboo. A total of 917 markers were successfully validated in 9 accessions with ~39.8% polymorphic potential. Retrieved from validated microsatellite markers, 23 markers were selected for polymorphic analysis among 78 accessions and 64 alleles were detected with an average of 2.78 alleles per primers. The cluster result indicated the majority of the accessions were consistent with their current taxonomic classification, confirming the suitability and effectiveness of the developed microsatellite markers. The variations of microsatellite marker in different species were confirmed by sequencing and in silico comparative genome mapping were investigated. Lastly, a bamboo microsatellites database ( http://www.bamboogdb.org/ssr ) was implemented to browse and search large information of bamboo microsatellites. Consequently, our results of microsatellite marker development are valuable for assisting bamboo taxonomy and investigating genomic studies in bamboo and related grass species.
机译：通过生殖器官的形态学分类法对竹类分类法有严格的限制，这主要是由于竹子的开花事件很少且不可预测。在这里，我们介绍了基于毛竹（Phyllostachys edulis）基因组的微卫星的第一个全基因组范围的分析和应用，以协助竹类分类。在鉴定出的127,593个微卫星重复基序中，设计了1,451个微卫星的引物，并将1,098个标记物物理定位在毛竹基因组上。共有917个标记已在9个品系中成功验证，具有约39.8％的多态性。从经过验证的微卫星标记中检索出来，在78个种质中选择了23个标记用于多态分析，检测到64个等位基因，每个引物平均有2.78个等位基因。聚类结果表明，大多数种质与它们目前的分类学分类是一致的，证实了开发的微卫星标记的适用性和有效性。通过测序证实了不同物种中微卫星标记的变异，并在计算机上比较了基因组作图。最后，建立了竹微卫星数据库（http://www.bamboogdb.org/ssr），以浏览和搜索竹微卫星的大量信息。因此，我们的微卫星标记开发结果对于协助竹类分类学和研究竹类及相关草种的基因组研究具有重要价值。

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