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Micro-RNAs, their target proteins, predispositions and the memory of filial imprinting

机译：微小RNA，其靶蛋白，易感性和孝子印迹的记忆

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Visual imprinting is a learning process whereby young animals come to prefer a visual stimulus after exposure to it (training). The intermediate medial mesopallium (IMM) in the domestic chick forebrain is critical for visual imprinting and contributes to molecular regulation of memory formation. We investigated the role of micro-RNAs (miRNAs) in such regulation. Twenty-four hours after training, miRNA spectra in the left IMM were compared between chicks with high preference scores (strong memory for imprinting stimulus), and chicks with low preference scores (weak memory for imprinting stimulus). Using criteria of significance and expression level, we chose gga-miR-130b-3p for further study and found that?down-regulation correlated with learning strength. No effect was detected in posterior nidopallium, a region not involved in imprinting. We studied two targets of gga-miR-130b-3p, cytoplasmic polyadenylation element binding proteins 1 (CPEB-1) and 3 (CPEB-3), in two subcellular fractions (P2 membrane-mitochondrial and cytoplasmic) of IMM and posterior nidopallium. Only in the left IMM was a learning-related effect observed, in membrane CPEB-3. Variances from the regression with preference score and untrained chicks suggest that, in the IMM, gga-miR-130b-3p level reflects a predisposition, i.e. capacity to learn, whereas P2 membrane-mitochondrial CPEB-3 is up-regulated?in a learning-specific way.
机译：视觉烙印是一个学习过程，幼小动物在接触视觉刺激（训练）后开始喜欢视觉刺激。家禽前脑的中间内侧中间皮层（IMM）对于视觉印迹至关重要，并有助于记忆形成的分子调控。我们研究了微RNA（miRNA）在这种调控中的作用。训练后二十四小时，比较了具有较高优先权得分（对印迹刺激记忆力强）和较低优先权分数（对印迹刺激记忆力弱）的雏鸡左IMM中的miRNA谱图。使用显着性和表达水平的标准，我们选择了gga-miR-130b-3p进行进一步研究，发现下调与学习强度相关。未检测到后部鼻pal区，该区不涉及印迹。我们研究了IMM和后壁鼻pal的两个亚细胞部分（P2膜-线粒体和细胞质）的两个gga-miR-130b-3p靶标，即胞质聚腺苷酸化元素结合蛋白1（CPEB-1）和3（CPEB-3）。仅在左侧的IMM中，在膜CPEB-3中观察到了学习相关的作用。偏好评分和未经训练的雏鸡的回归差异表明，在IMM中，gga-miR-130b-3p水平反映了易感性，即学习能力，而P2膜线粒体CPEB-3在学习中上调。特定的方式。

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