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首页> 外文期刊>Scientific reports. >Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules
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Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules

机译：级联确定性侧向位移模块模块化平台中生物颗粒的分离

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Deterministic lateral displacement (DLD) has been extensively implemented in the last decade for size-based sample preparation, owing to its high separation performances for a wide range of particle dimensions. However, separating particles from 1 μm to 10 μm in one single DLD device is challenging because of the required diversity of pillar dimensions and inherent fabrication issues. This paper presents an alternative approach to achieve the extraction of E. coli bacteria from blood samples spiked with prostate cancer cells. Our approach consists in cascading individual DLD devices in a single automated platform, using flexible chambers that successively collect and inject the sample between each DLD stage without any external sample manipulation. Operating DLD separations independently enables to maximize the sorting efficiency at each step, without any disturbance from downstream stages. The proposed two-step automated protocol is applied to the separation of three types of components (bacteria, blood particles and cancer cells), with a depletion yield of 100% for cancer cells and 93% for red blood cells. This cascaded approach is presented for the first time with two DLD modules and is upscalable to improve the dynamic range of currently available DLD devices.
机译：确定性横向位移（DLD）在过去的十年中已广泛用于基于尺寸的样品制备，这是由于其在各种粒径范围内的高分离性能。然而，由于所需的支柱尺寸多样性和固有的制造问题，在一个单一的DLD设备中将颗粒从1μm分离到10μm具有挑战性。本文提出了一种替代方法，可从掺有前列腺癌细胞的血液样本中提取大肠杆菌。我们的方法包括使用灵活的腔室在单个自动化平台中级联单个DLD设备，该腔室在每个DLD阶段之间连续收集和注入样品，而无需任何外部样品操作。独立运行DLD分离可以最大程度地提高每个步骤的分选效率，而不会受到下游阶段的干扰。拟议的两步自动化方案可用于分离三种类型的成分（细菌，血液颗粒和癌细胞），癌细胞的耗竭率为100％，红细胞的耗竭率为93％。首次使用两个DLD模块展示了这种级联方法，并且可以升级以提高当前可用DLD设备的动态范围。

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