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首页> 外文期刊>Journal of animal and veterinary advances >Characteristics of Proliferation and Differentiation-Dependent C/EBP, PPARγ and Leptin Gene Expression Patterns Intrinsic to Bovine Intramuscular, Perirenal and Subcutaneous Preadipocytes
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Characteristics of Proliferation and Differentiation-Dependent C/EBP, PPARγ and Leptin Gene Expression Patterns Intrinsic to Bovine Intramuscular, Perirenal and Subcutaneous Preadipocytes

机译：增殖和分化依赖性C / EBP，PPARγ和瘦素基因表达模式的特征是牛肌肉内，肝癌和皮下前脂肪细胞

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摘要

Adipose depotsite-specific variability in physiological characteristics of preadipocytes could be in part, intrinsic to the cells and become evident through in vitro proliferation and differentiation-dependent gene expression patterns in preadipocyte in the cell culture. This study was designed to characterize proliferation and differentiation-dependent gene expression patterns intrinsic to intramuscular, perirenal and subcutaneous preadipocytes. About 3 type preadipocytes are prepared using the ceiling culture method from bovine intramuscular, perirenal and are demonstrated subcutaneous adipose depots of a single Japanese Black steer. Adipose depot site-specific proliferation and differentiation-dependent gene expression patterns intrinsic to preadipocyte by comparing the mRNA levels of CCAAT enhancer binding protein C/EBPα, C/EBPβ, C/EBPδ, Peroxisome Proliferator-Activated Receptor γ (PPARγ) and leptin before and after the induction of differentiation among culture of the 3 type preadipocytes. These results may suggest the existence of the site-specific preadipocyte-intrinsic proliferation and differentiation program.
机译：前脂肪细胞的生理特性的脂肪蛋白质特异性可变性可以部分地，细胞内部，通过在细胞培养中的前脂肪细胞中的体外增殖和分化依赖性基因表达模式明显。本研究旨在表征增殖和分化依赖性基因表达模式，其内在肌内肌肉内，脑梗死和皮下前脂肪细胞。使用来自牛肌肉内，脑梗死的天花板培养方法制备约3型前脂肪细胞，并证明了单个日本黑色转向的皮下脂肪贮藏。通过比较CCAAT增强剂结合蛋白C /EBPα，C /EBPβ，C /EBPδ，过氧化物体增殖物激活受体γ（PPARγ）和瘦素，通过比较CCAAT增强剂结合蛋白C /EBPα，C /EBPβ，C /EBPδ，过氧化物体增殖物激活的受体γ（PPARγ）和瘦蛋白的mRNA水平，脂肪阶段特异性增殖和分化依赖性基因表达模式。并且在诱导3型前脂肪细胞的培养物中的诱导之后。这些结果可能表明存在特异性预脂肪细胞内在增殖和分化计划的存在。

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