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首页> 外文学位 >Differentiation of bovine intramuscular and subcutaneous preadipocytes.
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Differentiation of bovine intramuscular and subcutaneous preadipocytes.

机译：牛肌肉内和皮下前脂肪细胞的分化。

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摘要

Intramuscular (i.m.) and subcutaneous (s.c. ) adipose tissue substantially influence beef carcass value, and are of economic importance to the beef industry. Adipose development is at least partially controlled by the differentiation of preadipocytes into mature adipocytes. We hypothesized that bovine i.m. and s.c. preadipocyte differentiation would be enhanced by a peroxisome proliferator-activated receptor ( PPAR)-gamma agonist, troglitazone (TRO), and a glucocorticoid, dexamethasone (DEX), with the relative response being greater in i.m. than s.c. cells.; In the first set of experiments, preadipocytes from i.m. and s.c. stromal-vascular (S-V) cells of an Angus steer were cloned and used to optimize culture conditions supporting adipose differentiation, as well as compare the adipogenic responses of i.m. and s.c. preadipocytes to TRO. A higher percentage of isolated s.c. clones (47%) were identified as adipogenic than i.m. (12.5%) (P 0.001). A s.c. preadipocyte clone was used to optimize differentiation culture conditions. Addition of 10 and 20 muL/mL serum lipid (SL) to serum-free media containing 280 nM insulin increased glycerol-3-phosphate dehydrogenase (GPDH) activity. Inclusion of 1.25 to 10 muM TRO to media containing insulin and SL also increased GPDH activity (P 0.001). No GPDH activity was detected when medium included insulin, octanoate, and acetic acid, following 48 h exposure to DEX. However, insulin, SL, TRO, and DEX stimulated GPDH activity ( P 0.001). Omission of TRO or insulin from this media lowered GPDH activity (P 0.001), while removal of DEX tended to reduce activity (P = 0.06). When i.m. (n = 3) and s.c. (n = 2) clones were compared, all clones responded to addition of 20 to 60 muM TRO (P 0.02), with no depot differences (P = 0.47).; In the second set of experiments, i.m. and s.c. S-V cells isolated from three Angus steers were used to determine the adipogenic effects of DEX and TRO. Forty and 60 pM TRO increased GPDH activity 2.9- and 3.4-fold compared to non-treated cells (P 0.03), with no depot differences (P = 0.32). When DEX and TRO were tested in combination, DEX stimulated a 1.8-fold increase in GPDH activity (P = 0.006), while TRO induced a 2.5-fold increase in GPDH activity (P = 0.02). No DEX x TRO interaction (P = 0.53) or depot effects (P = 0.41) were found. In clonal analysis experiments, DEX increased the percentage of adipogenic colonies by 1.2-fold ( P = 0.02), while TRO increased the proportion of differentiated colonies by 2.3-fold (P = 0.01). No DEX x TRO interaction ( P = 0.65) or depot differences (P = 0.10) were found. However, the percentage of differentiated cells within adipogenic colonies was 6.4-fold greater in s.c. isolates than i.m. (P 0.001). Dexamethasone had no significant effect (P = 0.10) while TRO increased the proportion of differentiated cells by 10-fold (P 0.001). In summary, differentiation of bovine i.m. and s.c. preadipocytes was enhanced in response to SL, DEX, and TRO. Under identical media conditions, s.c. preadipocytes have a greater capacity to differentiate compared to i.m.
机译：肌内（i.m.）和皮下（s.c.）脂肪组织会显着影响牛肉car体的价值，并且对牛肉行业具有重要的经济意义。脂肪的发育至少部分受前脂肪细胞向成熟脂肪细胞分化的控制。我们假设那是牛和s.c.过氧化物酶体增殖物激活受体（PPAR）-γ激动剂曲格列酮（TRO）和糖皮质激素地塞米松（DEX）会增强前脂肪细胞的分化，而在i.m.比南卡罗来纳州细胞。;在第一组实验中，来自i.m.的前脂肪细胞和s.c.克隆了安格斯牛的基质-血管（S-V）细胞，用于优化支持脂肪分化的培养条件，并比较i.m的成脂反应。和s.c.前脂肪细胞转TRO。隔离的s.c.克隆（47％）被鉴定为成脂性比i.m. （12.5％）（P <0.001）。交流前脂肪细胞克隆用于优化分化培养条件。向包含280 nM胰岛素的无血清培养基中加入10和20μL/ mL的血清脂质（SL），可提高3-磷酸甘油脱氢酶（GPDH）的活性。在含有胰岛素和SL的培养基中加入1.25至10μMTRO也会增加GPDH活性（P <0.001）。暴露于DEX 48小时后，当培养基中包含胰岛素，辛酸酯和乙酸时，未检测到GPDH活性。但是，胰岛素，SL，TRO和DEX刺激了GPDH的活性（P <0.001）。从该培养基中省略TRO或胰岛素会降低GPDH活性（P <0.001），而去除DEX则会降低其活性（P = 0.06）。当我。 （n = 3）和s.c. （n = 2）个克隆进行了比较，所有克隆均响应添加20至60μMTRO（P <0.02），无贮库差异（P = 0.47）。在第二组实验中，和s.c.从三个安格斯牛分离的S-V细胞用于确定DEX和TRO的成脂作用。与未处理的细胞相比，四十和60 pM TRO的GPDH活性增加了2.9倍和3.4倍（P <0.03），没有储库差异（P = 0.32）。结合测试DEX和TRO时，DEX刺激了GPDH活性增加了1.8倍（P = 0.006），而TRO刺激了GPDH活性增加了2.5倍（P = 0.02）。没有发现DEX x TRO相互作用（P = 0.53）或仓库效应（P = 0.41）。在克隆分析实验中，DEX将成脂菌落的百分比提高了1.2倍（P = 0.02），而TRO将分化菌落的比例提高了2.3倍（P = 0.01）。没有发现DEX x TRO相互作用（P = 0.65）或仓库差异（P = 0.10）。但是，成脂菌落内分化细胞的百分数在s.c中要高6.4倍。比我更孤立（P <0.001）。地塞米松无明显作用（P = 0.10），而TRO使分化细胞的比例增加了10倍（P <0.001）。总而言之，牛i.m.和s.c.前脂肪细胞对SL，DEX和TRO的反应增强。在相同的媒体条件下，与i.m.相比，前脂肪细胞具有更大的分化能力。

著录项

  • 作者

    Grant, Aaron Christopher.;

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Agriculture Animal Culture and Nutrition.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 154 p.
  • 总页数 154
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 饲料;
  • 关键词

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