• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Symposium on the Nutrition of Herbivores(ISNH-7); 20070917-22; Beijing(CN) >Regulation of proliferation and differentiation of Luxi bovine intramuscular preadipocytes by propionate
【24h】

Regulation of proliferation and differentiation of Luxi bovine intramuscular preadipocytes by propionate

机译：丙酸对芦溪牛肌内前脂肪细胞增殖和分化的调控

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of the present study was to investigate the regulation of propionate on the proliferation and differentiation of cultured intramuscular preadipocytes from three adult Luxi Yellow steers. The flow cytometry, lipid droplets stained by oil red O, and cytoplasmic fat content were detected after cultured cells were treated with different concentrations of propionate (0, 1, 3, 6 mM). The results showed that the separated cells were highly homogeneous and proliferative. Propionate (1 mM) did not significantly affect (P＞0.05) the cell proportion of phase G0/G1, but remarkably (P＜0.05) increased the proportion of phase S+G2/M. However, the cell proportion significantly increased (P＜0.05) in the phase G0/G1, and decreased (P＜0.05) in the phase S+G2/M when the concentration of propionate reached to 6 mM. When the confluent intramuscular preadipocytes were treated with insulin (10 μg/ml) and dexamethasone (0.25 μM), small lipid droplets began to appear, and their number rapidly increased around the nuclei on day 6. Subsequently, when the confluent intramuscular preadipocytes were treated with propionate, significant accumulation of lipid droplets was observed on day 6, and lipid accumulation extent was dependent on the dose of propionate. In addition, cytoplasmic fat contents of cultured preadipocytes treated with propionate (1, 3, 6 mM) for 6 days were 1.5,2, and 2 times higher than those of control group (without propionate addition). In conclusion, the present study confirmed that there were indeed functionally active preadipocytes existing in the muscle of Chinese adult Luxi steers. Propionate may play an important physiological role in regulation of adipogenesis and stimulation of proliferation and differentiation of Luxi bovine intramuscular preadipocytes.
机译：本研究的目的是研究丙酸酯对来自三个成年Lux西黄牛的肌肉内前脂肪细胞增殖和分化的调节。用不同浓度的丙酸（0、1、3、6 mM）处理培养的细胞后，检测流式细胞仪，油红O染色的脂滴和细胞质脂肪含量。结果表明，分离的细胞是高度均质的和增殖的。丙酸（1 mM）对G0 / G1期细胞比例无明显影响（P＞ 0.05），但显着（P ＜0.05）增加了S + G2 / M期细胞的比例。然而，当丙酸盐浓度达到6 mM时，细胞比例在G0 / G1期显着增加（P ＜0.05），在S + G2 / M期降低（P ＜0.05）。当用胰岛素（10μg/ ml）和地塞米松（0.25μM）处理融合的肌内前脂肪细胞时，小脂质滴开始出现，并且其数量在第6天迅速在核周围增加。随后，当处理融合的肌内前脂肪细胞时，在丙酸的情况下，在第6天观察到大量的脂质滴积累，并且脂质的积累程度取决于丙酸的剂量。此外，用丙酸盐（1、3、6 mM）处理6天的培养前脂肪细胞的细胞质脂肪含量是对照组（不添加丙酸盐）的1.5、2和2倍。总而言之，本研究证实了中国成年鲁西ste牛肌肉中确实存在功能活跃的前脂肪细胞。丙酸酯可能在调控脂肪形成以及刺激Lux西牛肌内前脂肪细胞增殖和分化中起重要的生理作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号