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Effects of MicroRNA-143 in the differentiation and proliferation of bovine intramuscular preadipocytes

机译：MicroRNA-143对牛肌内前脂肪细胞分化和增殖的影响

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摘要

MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. miRNA-143 has been proposed to play a role in the differentiation of preadipocytes. However, effects and mechanism of miRNA-143 in the differentiation of mammals intramuscular adipocytes is unknown. In this study, the fibroblast-like preadipocytes were cultured from the marbling muscle tissue of holstein steers by the ceiling culture method. The in vitro studies showed that the fibroblast-like preadipocytes could differentiated into mature adipocytes with up-regulated expression of miRNA-143. Furthermore, the transfection of the fibroblast-like preadipocytes with miRNA-143 antisense inhibitor induced a significant suppression of differentiation, and indicated by decreased storage of lipid droplets and down-regulated expression of key adipocytes regulatory genes such as CCAAT/enhancer-binding protein-α (C/EBPα) and fatty acid binding proteins-4 (FABP-4). On the contrary, cells proliferation were increased with miRNA-143 inhibitor transfection. Taken together, our study provide the first evidence for stimulation of endogenous miRNA-143 in the differentiation of bovine intramuscular fat, which in part contribute to the regulated expression of adipocyte genes.
机译：MicroRNA（miRNA）是短的非编码RNA，可在转录后调节基因表达。已经提出miRNA-143在前脂肪细胞的分化中起作用。但是，miRNA-143在哺乳动物肌内脂肪细胞分化中的作用和机制尚不清楚。在这项研究中，通过顶培养法从荷斯坦牛的大理石花纹肌肉组织中培养成纤维样前脂肪细胞。体外研究表明，成纤维样前脂肪细胞可以分化为成熟的脂肪细胞，miRNA-143的表达上调。此外，用miRNA-143反义抑制剂转染成纤维细胞样前脂肪细胞可显着抑制分化，并表现为脂质液滴储存减少和关键脂肪细胞调控基因（如CCAAT /增强子结合蛋白- α（C /EBPα）和脂肪酸结合蛋白4（FABP-4）。相反，miRNA-143抑制剂转染可增加细胞增殖。综上所述，我们的研究提供了在牛肌内脂肪分化中刺激内源性miRNA-143的第一个证据，这部分地有助于调节脂肪细胞基因的表达。

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