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首页> 外文期刊>Infection and immunity >The Escherichia coli K-12 gntP gene allows E. coli F-18 to occupy a distinct nutritional niche in the streptomycin-treated mouse large intestine.
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The Escherichia coli K-12 gntP gene allows E. coli F-18 to occupy a distinct nutritional niche in the streptomycin-treated mouse large intestine.

机译：大肠杆菌K-12 GNTP基因允许大肠杆菌F-18在链霉素处理的小肠大肠中占据不同的营养地基。

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Escherichia coli F-18 is a human fecal isolate that makes type 1 fimbriae, encoded by the fim gene cluster, and is an excellent colonizer of the streptomycin-treated mouse intestine. E. coli F-18 fimA::tet, lacking type 1 fimbriae, was constructed by bacteriophage P1 transduction of the fim region of the E. coli K-12 strain ORN151, containing the tetracycline resistance gene from Tn10 inserted in the fimA gene, into E. coli F-18. E. coli F-18 fimA::tet was found to occupy a distinct niche in the streptomycin-treated mouse intestine when fed in small numbers (10(4) CFU) to mice, along with large numbers (10(10) CFU) of E. coli F-18, as defined by the ability of the E. coli F-18 fimA::tet strain to grow and colonize only 1 order of magnitude below E. coli F-18. The same effect was observed when mice already colonized with E. coli F-18 were fed small numbers of E. coli F-18 fimA::tet. Experiments which show that the E. coli K-12 gene responsible for this effect is not fim::tet but gntP, which maps immediately downstream of the fim gene cluster, are presented. gntP encodes a high-affinity gluconate permease, suggesting that the distinct niche in the mouse large intestine is defined by the presence of gluconate. The data presented here support the idea that small numbers of an ingested microorganism can colonize the intestine as long as it can utilize an available nutrient better than any of the other resident species can.
机译：大肠杆菌F-18是一种人的粪便分离物，其使由FIM基因簇编码的1型FIMBRIAE，并且是链霉素处理的小鼠肠道的优异殖民症。 E. COLI F-18 FIMA :: TET缺乏1型FIMBRIAE，由大肠杆菌K-12菌株ORN151的FIM区的噬菌体P1转导构成，含有来自TN10的四环素抗性基因插入FIMA基因，进入大肠杆菌F-18。 E. Coli F-18 FIMA :: TET被发现当用少量（10（4）CFU）喂食少数（10（10）CFU）大肠杆菌F-18，如E. Coli F-18 FIMA :: TET菌株生长的能力，并且在大肠杆菌F-18以下仅为1个级的殖民化。当用大肠杆菌F-18殖民殖民的小鼠喂食少量大肠杆菌F-18 FIMA :: Tet时，观察到相同的效果。实验表明，负责这种效果的大肠杆菌K-12基因不是FIM :: TET，但是GNTP，其立即下游的映射到FIM基因簇的下游。 GNTP编码高亲和力的葡萄糖酸盐允许，表明小鼠大肠中的不同的乳蛋白由葡萄糖酸盐的存在定义。这里呈现的数据支持少数摄入的微生物可以殖民肠道，只要它可以利用比任何其他驻留的物种都可以利用可用的营养物。

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