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首页> 外文期刊>Infection and immunity >Fatty acid composition of the major phospholipids of Pneumocystic carinii: comparison with those in the lungs of normal and methylprednisolone-immunosuppressed rats.
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Fatty acid composition of the major phospholipids of Pneumocystic carinii: comparison with those in the lungs of normal and methylprednisolone-immunosuppressed rats.

机译：Pneumocystis carinii的主要磷脂的脂肪酸组成：与正常和甲基丙酮 - 免疫抑制大鼠肺的脂肪酸组成。

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Large numbers of viable organisms can be isolated from the corticosteroid-immunosuppressed rat model of Pneumocystis carinii pneumonia. With the development of purification protocols that provide organism preparations of high purity, meaningful lipid biochemical analyses of this important opportunistic pathogen can now be conducted. The phospholipid class composition of the pathogen was reported earlier, together with observations of changes that occur in the rat lungs in response to methylprednisolone immunosuppression treatment. In this report, analyses of the effects of corticosteroids on the fatty acid compositions of the major lung phospholipids, individually isolated and purified by thin-layer chromatography, were elucidated and quantified by gas-liquid chromatography. In response to methylprednisolone, there was a relative increase in palmitate and there were decreases in several unsaturated fatty acids of the rat whole-lung total polar lipids leading to a doubling of the saturation index. Reciprocal changes in the relative concentrations of palmitate and stearate in phosphatidylethanolamine, phosphatidylinositol, lysophosphatidylcholine, and cardiolipin were observed, suggesting that there is tight control of acylation of these phospholipids in the lung. Detailed phospholipid fatty acid analyses were also performed with mixed life cycle stages of P. carinii organisms. The most abundant phospholipids, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol, had much higher concentrations of oleic acid and lower concentrations of palmitate in P. carinii than in lung tissue. Sphingomyelin in lung tissue and P. carinii differed from the glycerophospholipids by the presence of high levels of saturated C(22) and C(24) fatty acids. This study represents the most comprehensive fatty acid analysis of rat lung phospholipids and the changes that occur in response to corticosteroid treatment. It is the first report about the fatty acids of individual phospholipids of the opportunistic protist P. carinii carinii.
机译：从肺炎肺肺炎肠道肺炎的皮质类固醇免疫抑制大鼠模型中可以分离大量的活生物体。随着在提供高纯度的有机体制剂的纯化方案的发展，现在可以进行这种重要机会化病原体的有意义的脂质生化分析。先前报道了病原体的磷脂类组合物，以及对大鼠肺的变化的观察结果响应于甲基己酮醇免疫抑制处理。在本报告中，通过气液色谱法阐明并定量了通过气液色谱法阐明和量化皮质类固醇对主要肺磷脂的脂肪酸组成的影响，分析了通过薄层色谱分离和纯化。响应于甲基己酮醇，棕榈酸盐的相对升高，大鼠全肺总极性脂质的几种不饱和脂肪酸中的降低，导致饱和指数的加倍。观察磷脂酰乙醇胺，磷脂酰肌醇，溶血磷脂胺和心磷脂中相对浓度的相对浓度的相互变化，表明肺中这些磷脂的酰化存在紧密控制。还用P. Carinii生物的混合生命周期阶段进行详细的磷脂脂肪酸分析。最丰富的磷脂，磷脂酰胆碱，磷脂酰乙醇胺和磷脂酰肌醇，在P. Carinii中具有更高的油酸和较低浓度的棕榈酸根，而不是肺组织。肺组织中的鞘豆苷和P. Carinii通过高水平的饱和C（22）和C（24）脂肪酸不同于甘油磷脂。该研究代表了大鼠肺磷脂的最全面的脂肪酸分析及响应皮质类固醇治疗而发生的变化。这是关于机会型物质P. Carinii Carinii的单个磷脂脂肪酸的第一份报告。

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