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首页> 外文期刊>Infection and immunity >Cloning, Expression, and Sequencing of a Cell Surface Antigen Containing a Leucine-Rich Repeat Motif from Bacteroides forsythus ATCC 43037
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Cloning, Expression, and Sequencing of a Cell Surface Antigen Containing a Leucine-Rich Repeat Motif from Bacteroides forsythus ATCC 43037

机译：细胞表面抗原的克隆，表达和测序含有富含亮氨酸的重复基序的细胞表面抗菌剂43037

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Bacteroides forsythus is a recently recognized human periodontopathogen associated with advanced, as well as recurrent, periodontitis. However, very little is known about the mechanism of pathogenesis of this organism. The present study was undertaken to identify the surface molecules of this bacterium that may play roles in its adherence to oral tissues or triggering of a host immune response(s). The gene (bspA) encoding a cell surface-associated protein of B. forsythus with an apparent molecular mass of 98 kDa was isolated by immunoscreening of a B. forsythus gene library constructed in a lambda ZAP II vector. The encoded 98-kDa protein (BspA) contains 14 complete repeats of 23 amino acid residues that show partial homology to leucine-rich repeat motifs. A recombinant protein containing the repeat region was expressed inEscherichia coli, purified, and utilized for antibody production, as well as in vitro binding studies. The purified recombinant protein bound strongly to fibronectin and fibrinogen in a dose-dependent manner and further inhibited the binding of B. forsythus cells to these extracellular matrix (ECM) components. In addition, adult patients with B. forsythus-associated periodontitis expressed specific antibodies against the BspA protein. We report here the cloning and expression of an immunogenic cell surface-associated protein (BspA) of B. forsythus and speculate that it mediates the binding of bacteria to ECM components and clotting factors (fibronectin and fibrinogen, respectively), which may be important in the colonization of the oral cavity by this bacterium and is also a target for the host immune response.
机译： bacterythus forsythus 是最近认可的人类牙周病病，与先进，以及复发，牙周炎。然而，关于该生物体发病机制的机制很少。本研究鉴定了这种细菌的表面分子，其可在其粘附到口腔组织或托管免疫应答的触发中起作用的作用。编码 B的细胞表面相关蛋白的基因（ BSPA ）。通过免疫围绕A B分离出明显的分子量的Forsythus 。 Forsythus 基因库在λzap II载体中构建。编码的98-KDA蛋白（BSPA）含有14个完全重复的23个氨基酸残基，其显示出富含亮氨酸的重复基序的部分同源性。含有重复区域的重组蛋白在大肠杆菌中表达，纯化，用于抗体产生，以及体外结合研究。纯化的重组蛋白以剂量依赖性方式强烈地与纤连蛋白和纤维蛋白原相结合，并进一步抑制 B的结合。 FORSYTHUS 细胞对这些细胞外基质（ECM）组分。此外，成年患者 B. Forsythus - 分配的牙周炎表达了针对BSPA蛋白的特异性抗体。我们在此报告 B的免疫原性细胞表面相关蛋白（BSPA）的克隆和表达。 FORSYTHUS 并推测它介导细菌对ECM组分和凝血因子（分别纤维蛋白和纤维蛋白原）的结合，这在该细菌的口腔定植中可能是重要的，并且也是主体的靶标免疫反应。

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