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首页> 外文期刊>Molecular and Cellular Biology >Rpm2p, a Component of Yeast Mitochondrial RNase P, Acts as a Transcriptional Activator in the Nucleus
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Rpm2p, a Component of Yeast Mitochondrial RNase P, Acts as a Transcriptional Activator in the Nucleus

机译：RPM2P，酵母线粒体RNaseP的组分，作为细胞核中的转录活化剂

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Rpm2p, a protein subunit of yeast mitochondrial RNase P, has another function that is essential in cells lacking the wild-type mitochondrial genome. This function does not require the mitochondrial leader sequence and appears to affect transcription of nuclear genes. Rpm2p expressed as a fusion protein with green fluorescent protein localizes to the nucleus and activates transcription from promoters containing lexA-binding sites when fused to a heterologous DNA binding domain, lexA. The transcriptional activation region of Rpm2p contains two leucine zippers that are required for transcriptional activation and are conserved in the distantly related yeast Candida glabrata. The presence of a mitochondrial leader sequence does not prevent a portion of Rpm2p from locating to the nucleus, and several observations suggest that the nuclear location and transcriptional activation ability of Rpm2p are physiologically significant. The ability of RPM2 alleles to suppress tom40-3, a temperature-sensitive mutant of a component of the mitochondrial import apparatus, correlates with their ability to transactivate the reporter genes with lexA-binding sites. In cells lacking mitochondrial DNA, Rpm2p influences the levels of TOM40, TOM6, TOM20, TOM22, and TOM37 mRNAs, which encode components of the mitochondrial import apparatus, but not that of TOM70 mRNA. It also affects HSP60 and HSP10 mRNAs that encode essential mitochondrial chaperones. Rpm2p also increases the level of Tom40p, as well as Hsp60p, but not Atp2p, suggesting that some, but not all, nucleus-encoded mitochondrial components are affected.
机译：RPM2P，酵母线粒体RNaseP的蛋白质亚基具有另一种功能，该功能对于缺乏野生型线粒体基因组的细胞是必不可少的。该功能不需要线粒体前导序列，并且似乎影响核基因的转录。 RPM2P表示为具有绿色荧光蛋白的融合蛋白，该荧光蛋白定位于核，并激活含有Lexa结合位点的启动子的转录，当融合到异源DNA结合结构域，Lexa时。 RPM2P的转录激活区域含有转录激活所需的两个亮氨酸拉链，并在远处相关的酵母 Candida Glabrata 中保守。线粒体前导序列的存在不会阻止rpm2p的一部分从定位到细胞核，并且若干观察结果表明RPM2P的核位置和转录激活能力在生理学上显着。 rpm2 等位基因来抑制线粒体进口设备的组分的温度敏感突变体的能力与它们将报告基因与雷克萨转移的能力相关联 - 绕线网站。在缺乏线粒体DNA的细胞中，RPM2P影响 Tom40 ， Tom6 ， Tom20 ， Tom22 ，以及 Tom37 MRNA，其编码线粒体进口设备的组件，但不是 TOM70 mRNA的组件。它还影响编码基本线粒体伴侣的 HSP60 和 HSP10 MRNA。 RPM2P还增加了TOM40P的水平，以及HSP60P，但不是ATP2P，表明有些但不是全部，核编码的线粒体组分受到影响。

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