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首页> 外文期刊>Journal of Clinical Microbiology >Sensitive microculture method for isolation of human immunodeficiency virus type 1 from blood leukocytes.
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Sensitive microculture method for isolation of human immunodeficiency virus type 1 from blood leukocytes.

机译：从血白细胞中分离人免疫缺陷病毒1型的敏感微培养方法。

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A study was conducted to compare our standard culture with a new microculture procedure for isolation of human immunodeficiency virus type 1 (HIV-1) from blood leukocytes. A total of 137 blood specimens from 102 HIV-1 antibody-positive individuals (52 were asymptomatic, 31 were symptomatic, and 19 had AIDS) were cultured in a microculture system in which 10(6) of the patients' peripheral blood mononuclear cells (PBMC) were cocultured with 10(6) phytohemagglutinin (PHA)-stimulated PBMC from an HIV-1 antibody-negative blood donor in 1.2 ml of culture medium. Results were compared with those of a historical control group of 139 standard HIV-1 cultures from 108 HIV-1 antibody-positive subjects (58 were asymptomatic, 36 were symptomatic, and 14 had AIDS). For standard cultures, 10 x 10(6) of the patients' PBMC were cocultured with 5 x 10(6) PHA-stimulated PBMC from an HIV-1 antibody-negative blood donor in 15 ml of culture medium. HIV-1 was isolated in 128 (93%) microcultures and 133 (96%) standard cultures. Both methods identified more than 75% of the positive cultures within 7 days and 100% of the positive cultures within 14 days. The isolation rates for HIV-1 in microcultures compared with standard cultures were 91 versus 93% (specimens from asymptomatic individuals), 93 versus 96% (specimens from symptomatic individuals), and 97 versus 100% (specimens from patients with AIDS). The median time to positivity for both culture methods was 7 days, and this correlated significantly with symptoms and CD4+ cell counts. The microculture method is a sensitive and less expensive system for isolation of HIV-1 from PBMC of HIV-1 antibody-positive individuals, and we recommend it as the culture method of choice, especially for children and patients with AIDS and severe anemia or leukopenia whose blood volume is an important consideration.
机译：进行了一项研究以将标准培养与新的微培养程序进行比较，以分离来自血白细胞的人类免疫缺陷病毒1型（HIV-1）。在102个HIV-1抗体阳性个体（52是无症状，31例有症状，19例艾滋病）的总共培养了137个血液标本在微循血系统中培养，其中10（6）例患者的外周血单核细胞（ PBMC）与来自培养基的HIV-1抗体阴性血液供体中的10（6）植物血糖素（PHA）刺激的PBMC共同化。结果与来自108个HIV-1抗体阳性受试者的139个标准HIV-1培养物的历史对照组的结果进行了比较（58是无症状的，36例有症状，14例艾滋病）。对于标准培养物，10×10（6）例患者的PBMC与来自HIV-1抗体阴性血液供体中的5×10（6）个PHA刺激的PBMC中的15mL培养基。 HIV-1分离在128（93％）微量培养和133（96％）标准培养物中分离。两种方法在7天内鉴定了75％以上的阳性培养物，在14天内获得100％的阳性培养物。与标准培养物相比的微幼苗中HIV-1的分离率为91，与93％（来自无症状的样品），93与96％（来自症状性质的标本），97种与100％（来自艾滋病患者的标本）。培养方法两种阳性的中值时间为7天，并且这种情况与症状和CD4 +细胞计数显着相关。微血液方法是一种敏感且更昂贵的系统，用于从HIV-1抗体阳性个体的PBMC中分离HIV-1，并推荐它作为培养方法的选择，特别是儿童和艾滋病患者和患有艾滋病和严重贫血或白癜风患者血量是重要的考虑因素。

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