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首页> 外文期刊>Scientific reports. >1H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization
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1H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization

机译:1H检测到在生物悬浮二氧化硅中捕获的蛋白质的固态NMR:一种用于生物材料表征的新工具

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Proton-detection in solid-state NMR, enabled by high magnetic fields (18 T) and fast magic angle spinning (50?kHz), allows for the acquisition of traditional (1)H-(15)N experiments on systems that are too big to be observed in solution. Among those, proteins entrapped in a bioinspired silica matrix are an attractive target that is receiving a large share of attention. We demonstrate that (1)H-detected SSNMR provides a novel approach to the rapid assessment of structural integrity in proteins entrapped in bioinspired silica.
机译:固态NMR中的质子检测,由高磁场(> 18t)和快速魔法角度旋转(>50ΩkHz),允许采集传统(1)H-(15)N实验在解决方案中太大了。其中,捕获在生物悬浮的二氧化硅基质中的蛋白质是一种有吸引力的靶,可接受大量的关注。我们证明(1)H检测到的SSNMR提供了一种新的方法,可以快速评估生物悬浮二氧化硅中捕获的蛋白质中的结构完整性。

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