...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Scientific reports. >Anti-melanoma activity of Forsythiae Fructus aqueous extract in mice involves regulation of glycerophospholipid metabolisms by UPLC/Q-TOF MS-based metabolomics study
【24h】

Anti-melanoma activity of Forsythiae Fructus aqueous extract in mice involves regulation of glycerophospholipid metabolisms by UPLC/Q-TOF MS-based metabolomics study

机译：小鼠抗黑色素瘤活性炭含水提取物涉及通过UPLC / Q-TOF基于MS的代谢物学研究调节甘油磷脂代谢

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Metabolomics is a comprehensive assessment of endogenous metabolites of a biological system in a holistic context. In this study, we evaluated the in vivo anti-melanoma activity of aqueous extract of Forsythiae Fructus (FAE) and globally explored the serum metabolome characteristics of B16-F10 melanoma-bearing mice. UPLC/Q-TOF MS combined with pattern recognition approaches were employed to examine the comprehensive metabolic signatures and differentiating metabolites. The results demonstrated that FAE exhibited remarkable antitumor activity against B16-F10 melanoma in C57BL/6 mice and restored the disturbed metabolic profile by tumor insult. We identified 17 metabolites which were correlated with the antitumor effect of FAE. Most of these metabolites are involved in glycerophospholipid metabolisms. Notably, several lysophosphatidylcholines (LysoPCs) significantly decreased in tumor model group, while FAE treatment restored the changes of these phospholipids to about normal condition. Moreover, we found that lysophosphatidylcholine acyltransferase 1 (LPCAT1) and autotaxin (ATX) were highly expressed in melanoma, and FAE markedly down-regulated their expression. These findings indicated that modulation of glycerophospholipid metabolisms may play a pivotal role in the growth of melanoma and the antitumor activity of FAE. Besides, our results suggested that serum LysoPCs could be potential biomarkers for the diagnosis and prognosis of melanoma and other malignant tumors.
机译：代谢组学是在整体背景下全面评估生物系统内源性代谢物。在这项研究中，我们评估了连翘（FAE）的含水提取物（FAE）的体内抗黑色素瘤活性，全球探索了B16-F10黑色素瘤小鼠的血清代谢特征。使用UPLC / Q-TOF MS与模式识别方法相结合，以检查综合代谢签名和分化代谢物。结果表明，FAE在C57BL / 6小鼠中对B16-F10黑色素瘤的表现出显着的抗肿瘤活性，并通过肿瘤损伤恢复了干扰的代谢概况。我们确定了17种代谢物，其与Fae的抗肿瘤效应相关。这些代谢物中的大多数参与甘油磷脂代谢。值得注意的是，在肿瘤模型组中，几种溶血磷脂酰胆碱（Lysopcs）显着降低，而Fae治疗将这些磷脂的变化恢复到正常情况下。此外，我们发现溶血磷脂酰胆碱酰基转移酶1（LPCAT1）和自吸引力（ATX）在黑素瘤中高度表达，并且FAE明显下调其表达。这些发现表明，甘油磷脂代谢的调节可能在黑素瘤的生长和FAE的抗肿瘤活性方面发挥枢转作用。此外，我们的结果表明，血清溶血性可能是对黑色素瘤和其他恶性肿瘤的诊断和预后的潜在生物标志物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号