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首页> 外文期刊>Cancer Management and Research >LncRNA LEF1-AS1 Promotes Ovarian Cancer Development Through Interacting with miR-1285-3p
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LncRNA LEF1-AS1 Promotes Ovarian Cancer Development Through Interacting with miR-1285-3p

机译：LNCRNA LEF1-AS1通过与MIR-1285-3P进行互动促进卵巢癌发展

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Background: Growing evidence indicates that long noncoding RNA (lncRNA) is a group of important regulator in cancer development. However, the correlation between lncRNA and ovarian cancer remains elusive. Here, we aimed to investigate the roles of LEF1-AS1 in ovarian cancer progression. Methods: LEF1-AS1 expression was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Survival rate was analyzed by Kaplan-Meier method. Cell Counting Kit-8 (CCK8) and colony formation assays were used for proliferation analysis. Transwell assay was utilized for analyses of migration and invasion. Luciferase reporter assay was performed to test the interaction between LEF1-AS1 and miR-1285-3p. Results: We showed that LEF1-AS1 expression was upregulated in ovarian cancer tissues compared with normal tissues. Besides, LEF1-AS1 level was positively correlated with lymph node metastasis and advanced stage. Enhanced expression of LEF1-AS1 may predict a poor prognosis. Moreover, LEF1-AS1 knockdown suppressed ovarian cancer cell proliferation, migration and invasion. Mechanistically, LEF1-AS1 exerted its oncogenic functions through interacting with miR-1285-3p to inhibit miRNA activity. Rescue assay validated that miR-1285-3p inhibitors abrogated LEF1-AS1-silencer-caused suppression of ovarian cancer progression. Conclusion: Our study revealed that LEF1-AS1 acts as a vital regulation in ovarian cancer progression.
机译：背景：日益增长的证据表明，长度非编码RNA（LNCRNA）是癌症发育中的一组重要调节因素。然而，LNCRNA和卵巢癌之间的相关性仍然难以捉摸。在这里，我们的目标是调查Lef1-AS1在卵巢癌进展中的作用。方法：通过定量实时聚合酶链反应（QRT-PCR）分析LEF1-AS1表达。通过Kaplan-Meier方法分析生存率。电池计数试剂盒-8（CCK8）和菌落形成测定用于增殖分析。 Transwell测定用于分析迁移和侵袭。进行荧光素酶报告器测定以测试LEF1-AS1和MIR-1285-3P之间的相互作用。结果：与正常组织相比，我们展示卵巢癌组织中的左旋1-AS1表达上调。此外，LEF1-AS1水平与淋巴结转移和高级阶段正相关。增强Lef1-AS1的表达可能预测预后差。此外，LEF1-AS1敲低抑制卵巢癌细胞增殖，迁移和侵袭。机械地，LEF1-AS1通过与miR-1285-3P相互作用以抑制miRNA活性来施加致癌功能。救援检验验证了MIR-1285-3P抑制剂废除偏出的LEF1-AS1-消声器引起的卵巢癌进展抑制。结论：我们的研究表明，Lef1-AS1作为卵巢癌进展的重要调节。

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