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首页> 外文期刊>African Journal of Biotechnology >Cloning and expression analysis of potassium channel gene NKT3 from Nicotiana tabacum
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Cloning and expression analysis of potassium channel gene NKT3 from Nicotiana tabacum

机译：烟草塔巴宫钾通道基因NKT3的克隆及表达分析

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摘要

Potassium (K+) is the predominant inorganic ion of plant cells. K+?channels in higher plant cells play an important role in regulating the influx and efflux of K+?from cells, and activity of these channels might be involved in plant stress resistance. A completely new K+?channel gene of?Nicotiana tabacum?was obtained through?homologous cloning strategy. The complete cDNA sequence was submitted to the?National Center for Biotechnology Information (NCBI) GenBank, designated as?NKT3?and the accession number is FJ230956. The?phylogenetic analysis indicated that?NKT3?is located at the branch of weak-inwardly rectifying K+?channels and might be a member of the Shaker family.?The?spatial and temporal expression of the gene was also investigated.?NKT3?is expressed abundantly in the roots, while little in the leaves of?N. tabacum. It might be involved in the process of K+?acquirement and release in tobacco roots.
机译：钾（K +）是植物细胞的主要无机离子。 K +？高等植物细胞的通道在调节K +的流入和流出中起重要作用，从细胞中，这些通道的活性可能涉及植物应激性。完全新的K +？尼古利亚纳塔卡突的通道基因？通过？同源克隆策略获得。完整的cDNA序列被提交给？国家生物技术信息（NCBI）Genbank中心，指定为？NKT3？和登录号是FJ230956。 ？系统发育分析表明？NKT3？位于弱于向内整流k + + + +的分支处，并且可能是振荡器家族的成员。同时也研究了该基因的动态和时间表达。？NKT3？大量表达在根部，虽然叶子很少？ Tabacum。它可能涉及K +的过程？在烟草根系中获取和释放。

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