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首页> 外文期刊>African Journal of Biotechnology >Characterization of four indigenous sheep breeds ofBalochistan, Pakistan by random amplified polymorphic DNAs
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Characterization of four indigenous sheep breeds ofBalochistan, Pakistan by random amplified polymorphic DNAs

机译：用随机扩增多晶晶型DNA表征俾路支山，巴基斯坦四个土着绵羊品种

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摘要

Genetic diversity among four indigenous breeds of sheep namely Mengali, Balochi, Beverigh and Harnai of Balochistan was detected by random amplified polymorphic DNA (RAPD) technique. Nineteen RAPD primers were initially applied and seventeen were chosen for further analysis, based on band pattern quality, reproducibility and presence of bands. An average of 92 RAPD fragments were obtained by using 17 primers and out of?the?36 fragments (39.13%) illustrated monomorphism while, 56 bands (60.87%) were polymorphic in all the four sheep breeds. The number of bands amplified in all the sheep breeds ranged from 2 to 10. The highest number of polymorphic loci 40 was observed in the Mengali breed, while the lowest 28 was in Balochi. Further, 33 and 31 polymorphic loci were seen in Beverigh and Harnai breeds, respectively. The overall gene diversity was highest in the Mengali (0.1474) while the lowest in Balochi breed (0.0998). Results of genetic similarities showed closer proximity between Balochi and Beverigh (0.992), Balochi and Harnai (0.992), and between Beverigh and Harnai (0.996). The resemblance was observed between Mengali and Balochi (0.918), between Mengali and Beverigh breeds (0.931) and between Mengali and Harnai breeds (0.925). The high level of genetic similarity between Balochi, Beverigh and Harnai sheep indicated the close relationship that might be due to common habitat. Further the present study highlighted the presence of diversity among and within breeds that can be used in the selection or crossbreeding programs of sheep. The present study suggests that RAPD-PCR can effectively be used to determine the genetic distances among the sheep breeds.
机译：通过随机扩增的多晶态DNA（RAPD）技术检测了四种土着品种绵羊中的羊皮，俾路支，贝尔米尔和Harnai的遗传多样性。初始施加19个RAPD引物，基于带状质量，可重复性和带的带状质量，重复性，选择十七种进一步分析。通过使用17个引物和掉α的平均水平的92个RAPD片段（39.13％）所示的单组分，其中56个带（60.87％）在所有四只羊品种中是多态性的。在所有绵羊品种中扩张的条带数量为2-10。在Mengali品种中观察到最多的多态基因座40，而最低的28位是俾路支。此外，分别在Beverigh和Harnai品种中看到33和31种多态性基因座。 Mengali（0.1474）中总体基因多样性最高，而Balochi品种中最低（0.0998）。遗传相似性的结果表明，Balochi和Beverigh（0.992），Balochi和Harnai（0.992），贝佛和Harnai（0.996）之间的近距离接近。孟加拉和俾路比（0.918）之间观察到相似性，蒙加利和贝尔韦尔疟品（0.931）和孟加拉和Harnai品种（0.925）之间。 Balochi，Beverigh和Harnai绵羊之间的高水平遗传相似性表明了可能是由于常见栖息地的密切关系。此外，本研究强调了可用于绵羊的选择或杂交方案中的品种中的多样性的存在。本研究表明，RAPD-PCR可以有效地用于确定绵羊品种中的遗传距离。

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