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Molecular traceability of the species origin of meats using multiplex PCR

机译：使用多重PCR的物种物种来源的分子可追溯性

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摘要

The objective of this study was the designing of a fast and reliable multiplex polymerase chain reaction (PCR) identification system for testing the pure and mixed species origin of meat samples. For conducting this research, different primers were designed for each species according to the conserved region of mitochondrial cytochrome b (Cytb) gene. The results revealed different specific amplified fragments of pure meat sources for buffalo, goat, cattle and sheep species. After mixing different portions of the mentioned meat sources, this method was able to trace less than 10% of the other species of meat in the mixture. Then, it can be concluded that this procedure is simple, cheap, rapid, and efficient, and so it can be used in the meat industry.
机译：本研究的目的是设计一种快速可靠的多重聚合酶链反应（PCR）识别系统，用于测试肉类样品的纯净和混合物种。为了进行该研究，根据线粒体细胞色素B（Cytb）基因的保守区域设计了针对每个物种的不同引物。结果显示了水牛，山羊，牛和羊群的纯肉类来源的不同特异性扩增碎片。在混合所述肉类源的不同部分后，该方法能够在混合物中追踪少于10％的其他肉。然后，可以得出结论，这个程序简单，便宜，快速，高效，因此它可以在肉类行业中使用。

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