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首页> 外文期刊>African Journal of Biotechnology >Molecular cloning and identification of tissue-specific expression of ent-kaurene oxidase gene in Momordica charantia
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Molecular cloning and identification of tissue-specific expression of ent-kaurene oxidase gene in Momordica charantia

机译：苦瓜牛肝菌菌胚胎氧化酶基因组织特异性表达的分子克隆与鉴定

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Momordica charantia?(bitter melon) is widely consumed as a vegetable and as a folk medicine. Ent-kaurene oxidase is a key enzyme of gibberellin (GA) synthesis by controlling the early GA biosynthesis. In this study, ent-kaurene oxidase cDNA sequence was successfully amplified from the total cDNA of pistillate flower buds, and a complete open reading frame (ORF) with 1563 bp, encoding 520 amino acids, containing 151 bp of 5’-untranslated region sequences and 217 bp of the 3’-untranslated region sequences was found. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed that the expression level of?M. charantia?ent-kaurene oxidase (McEKO) gene in pistillate flowers was significantly higher than in staminate flowers and neutral flowers, and the expression level was especially abundant in root and stem apexes. The gene was express in?Escherichia coli?cells with a molecular weight of 58.8 kD observed.
机译：苦瓜·布兰蒂亚？（苦瓜）被广泛消耗作为蔬菜和民间医学。 Ent-Kaurene氧化酶是通过控制早期的GA生物合成来合成胃蛋白酶（GA）合成的关键酶。在该研究中，从活泼的花芽的总cDNA成功扩增Ent-Kaurene氧化酶cDNA序列，以及具有1563bp的完全开放的读数框架（ORF），编码520氨基酸，含有151bp的5'-未旋转的区域序列发现了3'-未转过来的区域序列的217bp。定量实时聚合酶链反应（QRT-PCR）显示出表达水平的Δm。 Charantia？活泼的花朵中的Ent-Kaurene氧化酶（Mceko）基因显着高于雄制的花朵和中性花，表达水平尤其丰富根和茎尖。该基因在αscherichiacoli中表达，分子量为58.8kd的细胞。

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