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Chromium tolerance and reduction potential of Staphylococci species isolated from a fly ash dumping site in South Africa

机译:南非粉煤灰倾倒场地分离出葡萄球菌种类的铬耐受性和降低潜力

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In order to study the microbiology of chromium tolerance and reduction at a fly ash dumping site in South Africa, 15 core samples were investigated. It was shown that the 30 year old dumping site exhibited high concentrations of Cr (VI) ranging from 1.6 to 9.6 mg/g. From this contaminated fly ash dumping site, 67 chromium resistant bacteria (CRB) growing in liquid medium in presence of a high chromium concentration (500 μg/ml) were isolated. Full sequencing and phylogenetic analyses of the 16S rRNA gene allowed unambiguous identification of five?Staphylococcispecies distributed non-homogeneously through the depth profile of the site. There was a discrepancy in the way the five species tolerated chromium at 250 and 500 μg/ml in anaerobic conditions in the presence or absence of nitrate (KNO3). Their potential to reduce chromium under these conditions was also demonstrated.
机译:为了研究南非粉煤灰倾倒场地的铬耐受性和减少的微生物学,研究了15个核心样本。结果表明,30岁的倾倒网站表现出高浓度的Cr(VI),范围为1.6至9.6mg / g。从该污染的粉煤灰倾倒场所,分离出在高铬浓度(500μg/ ml)存在下生长在液体介质中的铬抗菌(CRB)。 16S rRNA基因的全部测序和系统发育分析允许无均匀地通过该部位的深度分布分布的五个β葡萄球菌的鉴定。在存在或不存在硝酸盐(KNO3)的情况下,在厌氧条件下在250和500μg/ ml处的五种耐溶于250和500μg/ ml的方式存在差异。还证明了它们在这些条件下减少铬的潜力。

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