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首页> 外文期刊>African Journal of Biotechnology >Improvement of Basmati rice (Oryza sativa L.) using traditional breeding technology supplemented with molecular markers
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Improvement of Basmati rice (Oryza sativa L.) using traditional breeding technology supplemented with molecular markers

机译：使用分子标记物补充的传统育种技术改善Basmati Rice（Oryza Sativa L.）

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摘要

The present study was undertaken to combine dwarfism (sd-1) from Pusa-1121 and aroma and resistance against diseases from Khalsa-7 into basmati background (Type-3). The dwarf segregants of the cross (Khalsa 7 X Pusa 1121) with Type-3 were screened under artificially created epiphytotic conditions in order to select plants free from common diseases of rice. Data recorded for yield and physical standards of quality acceptable in the global trade measured in All India Coordinated Trial Research Project (AICRP) trials were used for demonstration. In addition, two separate polymerase chain reaction (PCR) studies were conducted for detection of resistance against blast. PCR products using random amplified polymorphic DNA (RAPD) and sequence characterized amplified regions (SCAR) markers detected resistance against blast in the genotype Vallabh Basmati-21. The Basmati type, disease resistant and promising selections were assessed for lineage with traditional varieties of basmati rice. The resultant genotype VB-21 expressed close molecular lineage with traditional varieties of basmati rice as well. The present study indicated substantial utility of molecular markers to supplement traditional methods of breeding for improvement of basmati rice.
机译：本研究采用Pusa-1121和香气和抗Khalsa-7进入Basmati背景（3型）的香气和抗疾病的侏儒症（SD-1）。在人工产生的旁白核状病症下筛选交叉（Khalsa 7 X PUSA 1121）的矮种（Khalsa 7 x pusa 1121），以选择没有常见水稻疾病的植物。在所有印度协调的试验研究项目（AICRP）试验中，在全球贸易中获得的产量和物质质量标准的数据用于示范。此外，进行了两种单独的聚合酶链反应（PCR）研究以检测抗爆炸的抗性。使用随机扩增的多晶型DNA（RAPD）和序列表征扩增区域（瘢痕）标记的PCR产物检测到基因型Vallabh Basmati-21中的抗爆炸的抗性。利用传统品种的巴斯马蒂米饭，评估Basmati类型，抗病和有前途的选择。得到的基因型VB-21表达了与传统品种的Basmati米的密切分子谱系。本研究表明了分子标志物的实质性效用，以补充传统的育种方法来改善Basmati米。

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