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首页> 外文期刊>African Journal of Biotechnology >Detection and identification of Staphylococcus aureus in raw milk by hybridization to oligonucleotide microarray
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Detection and identification of Staphylococcus aureus in raw milk by hybridization to oligonucleotide microarray

机译：寡核苷酸微阵列杂交中原料乳金黄色葡萄球菌的检测与鉴定

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Staphylococcus aureus?causes foodborne diseases if consumed in contaminated milk products. Rapid detection and characterization of foodborne pathogen?S. aureus?is crucial for epidemiological investigations and food safety surveillance. It is still a challenge to detect and identify bacterial pathogens quickly and accurately according to the samples. In this study, we have amplified 16S rRNA of?S. aureusby specific primers, designed oligonucleotide probes, detected the sensitivity and specificity of the microarray assay, and also identified?S. aureus?in the raw milk samples by hybridization. The?S. aureus?and 2 control pathogens (Streptococcus suis?in pigs and?Shigella) were used for specificity of the microarray assay. Based upon the hybridization results, universal probes for bacterial pathogens,?S. aureusprobe,?Staphylococcus?spp. probe,?nucleic acid?fixture positive controls and positive experimental control showed positive signals with targeted?S. aureus. The samples were diluted from 101?to 106?cfu per ml for evaluating the sensitivity of the microarray assay. The levels were as high as 103?cfu per ml, all of the samples showed positive signals. This method for rapid and effective detection and identification of?S. aureus?in raw milk demonstrated high sensitivity and specificity.
机译：金黄色葡萄球菌？如果在受污染的奶制品中消耗，导致食源性疾病。食品载病原体的快速检测与表征。金黄色葡萄球菌？对于流行病学调查和食品安全监测至关重要。根据样品快速，准确地检测和鉴定细菌病原体仍然是一项挑战。在这项研究中，我们已经扩增了16S rRNA的Δs。 Aureusby特异性引物，设计的寡核苷酸探针，检测到微阵列测定的敏感性和特异性，并鉴定出Δs。金黄色葡萄球菌？在原料牛奶样品中通过杂交。 ？s。金黄色葡萄球菌？和2种病原体（Screptococcus suis？在猪和何种症中）用于微阵列测定的特异性。基于杂交结果，对细菌病原体的全身探针，ΔS。 aureusprobe，？葡萄球菌？spp。探针，？核酸？夹具阳性对照和阳性实验对照显示靶向αS的阳性信号。金黄色葡萄球菌。将样品从101℃稀释，每mL稀释至106. CFU，用于评估微阵列测定的敏感性。该水平高达103Ω/ ml，所有样品显示阳性信号。这种快速有效地检测和鉴定的方法。金黄色葡萄球菌？在生牛奶中表现出高敏感性和特异性。

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