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首页> 外文期刊>African Journal of Biotechnology >Medium optimization for acarbose production by Actinoplanes sp. A56 using the response surface methodology
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Medium optimization for acarbose production by Actinoplanes sp. A56 using the response surface methodology

机译：Actinoplanes SP的亚马斯糖产生的中等优化。 A56使用响应表面方法

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摘要

In order to improve the production of acarbose, the fermentation medium of acarbose-producing strain?Actinoplanes?sp. A56 was optimized in this paper. Firstly, fractional factorial design was employed to investigate the influences of glucose, maltose, corn steep liquor, soy bean flour and?monosodium glutamate?on acarbose production, and the results?revealed?that maltose and corn steep liquor had significant and positive effects. Then, the two significant factors used the method of the steepest ascent to reach the yield plateau of acarbose. To explore the best condition obtained by the steepest method, the concentrations of maltose and corn steep liquor were further optimized with central composite design and response surface analysis. As a result, the acarbose?production was increased from 837 to 1043 mg/L?by using this optimization strategy.
机译：为了改善氨基糖的生产，产生缺苗产生菌株的发酵培养基？辐射素αsp。 A56在本文中得到了优化。首先，采用分数阶乘设计来研究葡萄糖，麦芽糖，玉米陡液，大豆粉和谷氨酸钠的影响？在氨基糖生产和结果？揭示？麦芽糖和玉米陡液体具有显着且积极的效果。然后，两种重要因素使用了最陡上升的方法来达到Acarbose的产量高原。为了探讨最陡法获得的最佳条件，通过中央复合设计和响应面分析进一步优化了麦芽糖和玉米陡液的浓度。结果，通过使用这种优化策略，缺苗率从837增加到1043 mg / L.

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