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首页> 外文期刊>Agronomy Research >Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
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Isolation of streptomycetes causing common scab from 3-years old potato samples from South America

机译：从南美洲的3岁土豆样品中导致常见结痂的链霉菌霉菌分离

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In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative.
机译：在本文中，我们旨在评估来自旧土豆样品的抗菌菌的最佳条件。测试了一组培养基和预处理。选择最佳的是从秘鲁，智利和阿根廷分离来自土豆的actinobacteria。通过扩增相应的基因对硫氮霉毒素的存在测试分离物。将菌株的系统发育位置与其地理来源，致病性潜力和常见的CAAB（CS）症状存在于马铃薯样品中。我们证明，RNALATER可以成功地用于马铃薯果皮的长期保存，以便随后分离R2A培养基上的抗菌菌。许多链霉菌是染色体阳性的，尽管它们与描述导致Cs的病原体有关。诺卡迪亚本族属据报道是抗染素阳性的。潜在的致病菌株不仅来自感染的马铃薯而且来自缺乏Cs症状的人。 Scabby土豆的一些菌株是阴性阴性。

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