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首页> 外文期刊>Archaea: an international microbiological journal >Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation
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Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation

机译：新分离的几丁质酶产生菌株芽孢杆菌菌株的鉴定及表征用于几丁质降解

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Chitinases or chitinolytic enzymes have different applications in the field of medicine, agriculture, and industry. The present study is aimed at developing an effective hyperchitinase-producing mutant strain of novel Bacillus licheniformis. A simple and rapid methodology was used for screening potential chitinolytic microbiota by chemical mutagenesis with ethylmethane sulfonate and irradiation with UV. There were 16 mutant strains exhibiting chitinase activity. Out of the chitinase-producing strains, the strain with maximum chitinase activity was selected, the protein was partially purified by SDS-PAGE, and the strain was identified as Bacillus licheniformis (SSCL-10) with the highest specific activity of 3.4?U/mL. The induced mutation model has been successfully implemented in the mutant EMS-13 (20.2?U/mL) that produces 5-6-fold higher yield of chitinase, whereas the mutant UV-11 (13.3?U/mL) has 3-4-fold greater chitinase activity compared to the wild strain. The partially purified chitinase has a molecular weight of 66?kDa. The wild strain (SSCL-10) was identified as Bacillus licheniformis using 16S rRNA sequence analysis. This study explores the potential applications of hyperchitinase-producing bacteria in recycling and processing chitin wastes from crustaceans and shrimp, thereby adding value to the crustacean industry.
机译：几丁酶或依甲酸碱酶在医学，农业和工业领域有不同的应用。本研究旨在开发一种有效的高血管素酶产生的新型芽孢杆菌植物植物突变体菌株。使用乙基甲烷磺酸盐和用UV照射来使用简单且快速的方法来筛选潜在的几丁质微生物。有16个突变菌株表现出几丁酶活性。在几丁质酶产生菌株中，选择具有最大丁质酶活性的菌株，通过SDS-PAGE部分纯化蛋白质，菌株被鉴定为芽孢杆菌（SSCL-10），具有3.4μm的最高特异性活性。U / ml。诱导的突变模型已在突变体EMS-13（20.2μlmL）中成功地实施，其产生5-6倍的胰蛋白酶产率，而突变紫外线-11（13.3×u / ml）具有3-4 - 与野生菌株相比，更大的几丁质酶活性。部分纯化的几丁质酶的分子量为66Ωkda。使用16S rRNA序列分析鉴定野生菌株（Sscl-10）作为芽孢杆菌。本研究探讨了产生高血管素酶产生的细菌在甲壳类动物和虾的回收和加工甲壳素废物中的潜在应用，从而增加了甲壳类动物的价值。

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