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首页> 外文学位 >The isolation and identification of the organism(s) that cause 'Redglobe' table grape breakdown in California (Bacillus subtilis, Bacillus licheniformis, Hanseniaspora guilliermondii).
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The isolation and identification of the organism(s) that cause 'Redglobe' table grape breakdown in California (Bacillus subtilis, Bacillus licheniformis, Hanseniaspora guilliermondii).

机译：在加利福尼亚州（“枯草芽孢杆菌”，“地衣芽孢杆菌”，“ Hanseniaspora guilliermondii”）引起“ Redglobe”鲜食葡萄分解的生物体的分离和鉴定。

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摘要

In the fall of 2000, we first observed grapes with unusual symptoms. The symptoms consisted of individual and groups of adjacent berries with a watery appearance on the berry surface, disintegrated tissue, hairline cracks and wounds on the berries, flaccid berries, and the presence of “slip-skin.” Bacteria and yeasts cultured from the samples were isolated, purified, grouped, and screened for pathogenicity. One yeast (Hanseniaspora guilliermondii Pijper), and four bacteria (three were Bacillus subtilis and one was Bacillus licheniformis) were determined to be pathogenic, and were later identified. In all cases, infection occurred only in wound-inoculated berries. Inoculating wounded ‘Redglobe’ berries with different mixtures of purified microorganisms had a synergistic effect, resulting in larger lesions and greater incidence and severity of hairline and severe cracks compared to single isolate inoculations. Disease development occurred in wounded ‘Redglobe’ berries inoculated with a mix of purified microorganisms and incubated at both 0°C and 25°C.
机译：在2000年秋天，我们首先观察到葡萄具有异常症状。症状包括相邻浆果的个体和群体，在浆果表面上有水样外观，组织分解，浆果上发际线的裂缝和伤口，松软的浆果以及存在“滑皮”。从样品中培养的细菌和酵母菌被分离，纯化，分组并筛选致病性。确定一种酵母菌（ Hanseniaspora guilliermondii Pijper）和四种细菌（三种细菌是枯草芽孢杆菌，一种细菌是地衣芽孢杆菌）是致病的，并且后来被确定。在所有情况下，感染仅发生在伤口接种的浆果中。与单独的分离株接种相比，用不同的纯净微生物混合物接种受伤的“ Redglobe”浆果具有协同作用，从而导致更大的病斑，发际线和严重裂缝的发生率和严重性更高。病害发生在受伤的“ Redglobe”浆果中，混合了纯净的微生物，并在0°C和25°C的温度下孵育。

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  • 作者

    Basinal, Lisa Michelle.;

  • 作者单位

    California State University, Fresno.;

  • 授予单位 California State University, Fresno.;
  • 学科 Agriculture Plant Pathology.
  • 学位 M.S.
  • 年度 2003
  • 页码 44 p.
  • 总页数 44
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;
  • 关键词

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