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首页> 外文期刊>African Journal of Pharmacy and Pharmacology >Reverse phase high performance liquid chromatographic (HPLC) method for nimesulide tablets dosage form prepared for in vivo in vitro correlation (IVIVC) studies
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Reverse phase high performance liquid chromatographic (HPLC) method for nimesulide tablets dosage form prepared for in vivo in vitro correlation (IVIVC) studies

机译：反相高效液相色谱（HPLC）方法，用于体内相关（IVIVC）研究制备的尼美片剂剂型的尼美片剂剂型

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摘要

The cost effective and simple method of reverse phase high performance liquid chromatographic (HPLC) method was successfully developed for the assaying of nimesulide (NM) in tablets, formulated for?in vivo in vitro?correlation (IVIVC) studies. The assay consisted of isocratic elution of nimesulide in Hiber RT 254-4.6 Purospher STAR RP-18 column using mobile phase composed of acetonitrile, phosphate buffer (pH 5.5) and methanol in the ratio of 35:45:20, respectively. Nimesulide was detected at 230 nm at the flow rate of 1.4 ml/min. The method was found to be sensitive and linear in the range of 1 to 100 ng/ml with 0.9997 coefficient of correlation. Blank sample of mobile phase and placebo showed no interference with the peak of analyte at retention time of 7.93 min. All validation parameters were evaluated and were found within the acceptance limits. The simplicity and short run time makes the method more convenient for quantitative analysis and assay of tablets.
机译：成本效益和简单的反相高效液相色谱（HPLC）方法成功开发了用于测定片剂中的尼美（NM），用于在体外的体内配制出来的β中的β相关性（IVIVC）研究。该测定包括在H犯RT 254-4.6中的尼菊酯中的尼菊酯的等异构洗脱，其使用由乙腈，磷酸盐缓冲液（pH5.5）和甲醇分别为35:45:20的比例组成的流动相。以1.4ml / min的流速在230nm下检测尼菊酯。发现该方法在1至100ng / ml的范围内是敏感和线性，其相关系数为0.9997。流动相和安慰剂的空白样本显示出在7.93分钟的保留时间下对分析物的峰值没有干扰。所有验证参数都进行了评估，并在验收限制范围内找到。简单性和短的运行时间使得该方法更方便地进行片剂的定量分析和测定。

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