...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Evidence-based complementary and alternative medicine: eCAM >Purple Sweet Potato Leaf Extract Induces Apoptosis and Reduces Inflammatory Adipokine Expression in 3T3-L1 Differentiated Adipocytes
【24h】

Purple Sweet Potato Leaf Extract Induces Apoptosis and Reduces Inflammatory Adipokine Expression in 3T3-L1 Differentiated Adipocytes

机译：紫色甘薯叶提取物诱导细胞凋亡并减少3T3-L1分化脂肪细胞的炎症adipokine表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background. Purple sweet potato leaves (PSPL) are widely grown and are considered a healthy vegetable in Taiwan. PSPL contain a high content of flavonoids, and the boiling water-extracted PSPL (PSPLE) is believed to prevent metabolic syndrome. However, its efficacy has not yet been verified. Therefore, we investigated the effect of PSPLE on adipocytes.Methods. The differentiated 3T3-L1 cells used in this study were derived from preadipocytes that were differentiated into adipocytes using an adipogenic agent (insulin, dexamethasone, and 3-isobutyl-1-methylxanthine); approximately 90% of the cells were differentiated using this method.Results. Treating the differentiated 3T3-L1 cells with PSPLE caused a dose-dependent decrease in the number of adipocytes rather than preadipocytes. In addition, treatment with PSPLE resulted in apoptosis of the differentiated 3T3-L1 cells as determined by DAPI analysis and flow cytometry. PSPLE also increased the expression of cleaved caspase-3 and poly ADP-ribose polymerase (PARP). Furthermore, PSPLE induced downregulation of interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) gene expression in the differentiated 3T3-L1 cells.Conclusions. These results suggest that PSPLE not only induced apoptosis but also downregulated inflammation-associated genes in the differentiated 3T3-L1 cells.
机译：背景。紫色甘薯叶（PSPL）被广泛种植，在台湾被认为是一个健康的蔬菜。 PSPL含有高含量的黄酮类化合物，并且据信沸水提取的PSPL（PSPLE）可防止代谢综合征。但是，它的功效尚未得到验证。因此，我们研究了Psple对adipocytes.methods的影响。本研究中使用的分化的3T3-L1细胞衍生自使用脂肪型剂（胰岛素，地塞米松和3-异丁基-1-甲基黄嘌呤）分化成脂肪细胞的前脂肪细胞;使用该方法对大约90％的细胞进行分化。结果。用Psple治疗分化的3T3-L1细胞导致脂肪细胞数量的剂量依赖性降低而不是普通脂肪细胞。此外，通过DAPI分析和流式细胞术确定分化的3T3-L1细胞的细胞凋亡。 Psple还增加了切割的Caspase-3和聚ADP-核糖聚合酶（PARP）的表达。此外，Psple诱导在分化的3T3-L1细胞中的白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）基因表达的下调。结论。这些结果表明，Psple不仅诱导细胞凋亡，而且在分化的3T3-L1细胞中诱导细胞凋亡，而且还在下调的炎症相关基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号