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首页> 外文期刊>Genes and Nutrition >Conjugated and free sterols from black bean (Phaseolus vulgaris L.) seed coats as cholesterol micelle disruptors and their effect on lipid metabolism and cholesterol transport in rat primary hepatocytes
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Conjugated and free sterols from black bean (Phaseolus vulgaris L.) seed coats as cholesterol micelle disruptors and their effect on lipid metabolism and cholesterol transport in rat primary hepatocytes

机译：来自黑豆的共轭和游离甾醇（phoudolusulusulus l.）种子涂层作为胆固醇胶束破坏剂及其对大鼠原发性肝细胞脂质代谢和胆固醇运输的影响

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Phytosterols have been widely studied for their cholesterol-lowering effect. Conjugated phytosterol forms have been found more active than free moieties. There are no reports about the sterol profile of black bean seed coats neither its effects on cholesterol metabolism. The aim of this research was to identify and quantify phytosterols from black bean seed coats and to determine their effects on cholesterol micellar solubility and on mRNA and key protein levels involved in lipid/cholesterol metabolism and cholesterol transport in primary rat hepatocytes. Free phytosterols, acylated steryl glycosides, and steryl glycosides were extracted from black bean seed coats. They were identified through HPLC–MS–TOF and quantified through HPLC equipped with UV–visible and evaporative light-scattering detectors. Free and conjugated phytosterols from the coats significantly increased the inhibitory effect of cholesterol micelle formation compared with stigmasterol, which was used as control (P?
机译：植物甾醇已被广泛研究其胆固醇降低效果。已经发现共轭植物甾醇形式比自由部分更活跃。没有关于黑豆种子涂层的甾醇谱系的报告既不是对胆固醇代谢的影响。该研究的目的是鉴定和定量来自黑豆种子涂层的植物甾醇，并确定其对胆固醇胶质细胞和脂质/胆固醇代谢和原代大鼠肝细胞中胆固醇转运的mRNA和关键蛋白质水平的影响。从黑豆种子涂层中萃取游离植物甾醇，酰化斯蒂尔糖苷和斯蒂尔糖苷。通过HPLC-MS-TOF鉴定它们并通过配备紫外可见光和蒸发光散射探测器的HPLC定量。与Stigmasterol相比，来自涂层的自由且缀合的植物甾醇显着提高了胆固醇胶束形成的抑制作用，其用作对照（P≤0.05）。此外，黑豆种子涂层的植物甾醇通过在原代大鼠肝细胞中的脂质蛋白如甾醇调节元素结合蛋白1和脂肪酸合成（FAS）的下调减少了脂肪生成。关于β-氧化，植物甾醇上调了肉毒氨基棕榈酰转移酶I的表达并促进了长链脂肪酸的β-氧化。植物甾醇抑制胆固醇胶束溶解度并降低肝X受体的活化，降低肝脏Fas并促进长链脂肪酸的肝β-氧化。

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