...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >Genome-wide transcriptome profiling of the medicinal plant Zanthoxylum planispinum using a single-molecule direct RNA sequencing approach
【24h】

Genome-wide transcriptome profiling of the medicinal plant Zanthoxylum planispinum using a single-molecule direct RNA sequencing approach

机译：使用单分子直接RNA测序方法的药用植物Zanthoxylum Planispinum的基因组转录组分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

High-throughput RNA sequencing has revolutionized transcriptome-based studies of candidate genes, key pathways and gene regulation in non-model organisms. We analyzed full-length cDNA sequences in Zanthoxylum planispinum (Z. planispinum), a medicinal herb in major parts of East Asia. The full-length mRNA derived from tissues of leaf, early fruit and maturing fruit stage were sequenced using PacBio RSII platform to identify isoform transcriptome. We obtained 51,402 unigenes, with average 1781?bp per gene in 82.473?Mb gene lengths. Among 51,402, 3963 unigenes showed variety of isoform. By selection of one representative gene among each of the various isoforms, we finalized 46,306 unique gene set for this herb. We identified 76 cytochrome P450 (CYP450) and related isoforms that are of the wide diversity in the molecular function and biological process. These transcriptome data of Z. planispinum will provide a good resource to study metabolic engineering for the production of valuable medicinal drugs and phytochemicals.
机译：高通量RNA测序已彻底改变了非模型生物中候选基因，关键途径和基因调控的基于转录组的研究。我们在Zanthoxylum Planispinum（Z. Planispinum）中分析了全长cDNA序列，该药草在东亚主要部分的药草。使用PACBIO RSII平台对叶片，早期果实和成熟果汁组织的全长mRNA进行测序，以鉴定同种型转录组。我们获得了51,402个unigenes，平均为1781μl？每种基因为82.473μmbe≤MB基因长度。在51,402中，3963个unigenes显示各种同种型。通过在各种同种型中的每一个中选择一个代表性基因，我们最终确定了46,306个独特的基因为该草本植物。我们鉴定了76个细胞色素P450（CYP450）和相关同种型，其在分子功能和生物过程中具有广泛的多样性。这些转录组数据的Z.Planispinum将提供良好的资源，以研究制作有价值的药物和植物化学物质的代谢工程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号