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首页> 外文期刊>Genomics >Small RNA profiling from meiotic and post-meiotic anthers reveals prospective miRNA-target modules for engineering male fertility in sorghum
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Small RNA profiling from meiotic and post-meiotic anthers reveals prospective miRNA-target modules for engineering male fertility in sorghum

机译：减数分裂和后减数分裂的小RNA分析揭示了高粱工程男性生育的前瞻性miRNA-target模块

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Understanding male gametophyte development is essential to augment hybrid production in sorghum. Although small RNAs are known to critically influence anther/pollen development, their roles in sorghum reproduction have not been deciphered yet. Here, we report small RNA profiling and high-confidence annotation of microRNAs (miRNAs) from meiotic and post-meiotic anthers in sorghum. We identified 262 miRNAs (82 known and 180 novel), out of which 58 (35 known and 23 novel) exhibited differential expression between two stages. Out of 35 differentially expressed known miRNAs, 13 are known to regulate anther/pollen development in other plant species. We also demonstrated conserved spatiotemporal patterns of 21- and 24-nt phasiRNAs and their respective triggers, miR2118 and miR2275, in sorghum anthers as evidenced in other monocots. miRNA target identification yielded 5622 modules, of which 46 modules comprising 16 known and 8 novel miRNA families with 38 target genes are prospective candidates for engineering male fertility in grasses.
机译：了解雄性配子体发育对于高粱增强杂种生产至关重要。虽然已知小RNA批判性地影响花粉/花粉发育，但它们在高粱繁殖中的作用尚未被破译。在这里，我们向高粱中的减数分裂和后减数分裂症的小酒经分析，报告小型RNA分析和高信用注释。我们鉴定了262个miRNA（82名已知和180个新颖），其中58（35个已知和23个新颖）在两个阶段表现出差异表达。在35个差异表达的已知的miRNA中，已知13次调节其他植物物种中的花药/花粉​​发育。我们还展示了21-和24-NT phasirnas的保守时滞模式及其各自的触发器，MiR2118和MiR2275，如同在其他单焦点中所证明的高粱。 miRNA靶标鉴定产生5622个模块，其中包含16个具有38个靶基因的16个已知和8个新的miRNA家族的46个模块是用于工程在草丛中的男性生育能力的前瞻性候选者。

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