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首页> 外文期刊>Frontiers in Oncology >Detection of Merkel Cell Polyomavirus DNA in Serum Samples of Healthy Blood Donors
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Detection of Merkel Cell Polyomavirus DNA in Serum Samples of Healthy Blood Donors

机译：梅尔克尔细胞多瘤DNA在健康血液供体血清样本中的检测

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Merkel cell polyomavirus (MCPyV) has been detected in 80% of Merkel cell carcinomas (MCC). In the host, the MCPyV reservoir remains elusive. MCPyV DNA sequences were revealed in blood donor buffy coats. In this study, MCPyV DNA sequences were investigated in the sera ( n ?=?190) of healthy blood donors. Two MCPyV DNA sequences, coding for the viral oncoprotein large T antigen (LT), were investigated using polymerase chain reaction (PCR) methods and DNA sequencing. Circulating MCPyV sequences were detected in sera with a prevalence of 2.6% (5/190), at low-DNA viral load, which is in the range of 1–4 and 1–5?copies/μl by real-time PCR and droplet digital PCR, respectively. DNA sequencing carried out in the five MCPyV-positive samples indicated that the two MCPyV LT sequences which were analyzed belong to the MKL-1 strain. Circulating MCPyV LT sequences are present in blood donor sera. MCPyV-positive samples from blood donors could represent a potential vehicle for MCPyV infection in receivers, whereas an increase in viral load may occur with multiple blood transfusions. In certain patient conditions, such as immune-depression/suppression, additional disease or old age, transfusion of MCPyV-positive samples could be an additional risk factor for MCC onset.
机译：Merkel Cell PolyomaviRus（MCPyV）已在Merkel细胞癌（MCC）的80％中检测到。在主人中，麦PYV水库仍然难以捉摸。在血液供体巴氏涂层中揭示了McPyV DNA序列。在该研究中，在血清中研究了McPyV DNA序列（N？= 190）的健康献血者。使用聚合酶链式反应（PCR）方法和DNA测序，研究了用于病毒癌蛋白大T抗原（LT）的两种McPyV DNA序列。在血清中检测到循环McPyV序列，其在低DNA病毒载量下患病率为2.6％（5/190），其在1-4和1-5℃的范围内，通过实时PCR和液滴拷贝/μl数字PCR分别。在五个McPyV阳性样品中进行的DNA测序表明，分析的两个McPyV LT序列属于MKL-1菌株。血液供体血清中循环循环麦PYVLT序列。来自献血者的McPyV阳性样本可以代表接收器中McPyV感染的潜在车辆，而病毒载荷的增加可能发生多种血液输血。在某些患者条件下，例如免疫抑制/抑制，额外的疾病或晚年，MCPYV阳性样品的输血可能是MCC发作的额外危险因素。

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