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首页> 外文期刊>Microbiology >The mid genes of Rhizobium sp. strain TAL1145 are required for degradation of mimosine into 3-hydroxy-4-pyridone and are inducible by mimosine
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The mid genes of Rhizobium sp. strain TAL1145 are required for degradation of mimosine into 3-hydroxy-4-pyridone and are inducible by mimosine

机译：Rhizobium sp的中间基因。菌株Tal1145是将Mimosine降解成3-羟基-4-吡啶酮，并通过Mimosine诱导

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摘要

Mimosine is a toxin present in the tree-legume leucaena (Leucaena leucocephala), including its root nodules and the root exudates. The leucaena-nodulating Rhizobium sp. strain TAL1145 degrades mimosine (Mid+) and utilizes it as a source of carbon and nitrogen. Twelve TAL1145 mutants defective in mimosine degradation (Mid?) were made through Tn3Hogus, TnphoA or kanamycin-resistance-cassette insertions. A 5·0?kb PstI fragment of TAL1145, subcloned from a cosmid clone containing mid genes for mimosine degradation, complemented most of the Mid? mutants. Sequencing this fragment and the adjacent 0·9?kb PstI fragment identified five genes, midA, midB, midC, midD and midR, of which the first three genes encode ABC transporter proteins involved in mimosine uptake, while midD encodes an aminotransferase required for degrading mimosine into 3-hydroxy-4-pyridone, and midR is a regulatory gene encoding a LysR-type transcriptional activator. The location of MidA in the periplasm was shown by making two midA?:?:?phoA fusions, which made active alkaline phosphatase in the periplasm. The various mid?:?:?gus and midA?:?:?phoA fusions were inducible by mimosine, and a midD?:?:?gus fusion mutant showed β-glucuronidase activity in the leucaena nodules, indicating that midD is expressed in the nodules. Similarly, a midA?:?:?phoA fusion expressed alkaline phosphatase activity in the leucaena nodules, indicating that mimosine induces midA transcription in the bacteroids. mid genes are specific for the Mid+ strains of leucaena Rhizobium and are absent in strains of other Rhizobium, Sinorhizobium and Bradyrhizobium spp.
机译：Mimosine是Tree-Megume Leucaena（Leucaena Leucocephala）中存在的毒素，包括其根结节和根部渗出物。 leucaena-结节Rhizobium sp。应变Tal1145降解Mimosine（中+），并用作碳和氮的源。通过TN3Hogus，TNphoa或卡那霉素 - 抵抗盒插入，制造了12个Tal1145突变体缺陷的突变体（中间→）。 At1145的5·0？Kb Psti片段，从含有Mimosine降解的中间基因的粘性克隆亚克隆，互补的中间的大部分？突变体。测序该片段和相邻的0·9？KB PSTI片段鉴定了五种基因，MIDA，MIDB，MIDC，MIDD和MIDR，其中前三个基因编码参与Mimosine摄取的ABC转运蛋白，而MIDD编码氨基转移酶降解所需的氨基转移酶Mimosine分为3-羟基-4-吡啶酮，MIDR是编码Lysr型转录活化剂的调节基因。通过制作两个MIDA显示PENIPRASAS中的MIDA的位置？：？：？PHOA融合，使活性碱性磷酸酶在周质中。各种中旬？：？Gus和Mida？：？佛罗州佛兰融合由Mimosine和MIDD诱导？：？：？GUS融合突变体在Leucaena结节中显示出β-葡萄糖醛酸酶活性，表明米德表达了含量结节。同样，米饭？：？：？氏族融合表达了在Leucaena结节中表达了碱性磷酸酶活性，表明Mimosine诱导菌体中的MIDA转录。 MID基因对于Leucaena Rhizobium的中期+菌株特异，并且在其他根瘤菌，Sinorhizobium和Bradyrhizobium spp的菌株中不存在。

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