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首页> 外文期刊>Rasayan Journal of Chemistry >ANTIBACTERIAL EFFECT OF PROTEIN AND PROTEIN HYDROLYSATES ISOLATED FROM BACTERIA Enterobacter hormaechei ASSOCIATED WITH MARINE ALGAE Sargassum sp.
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ANTIBACTERIAL EFFECT OF PROTEIN AND PROTEIN HYDROLYSATES ISOLATED FROM BACTERIA Enterobacter hormaechei ASSOCIATED WITH MARINE ALGAE Sargassum sp.

机译：蛋白质和蛋白质水解素的抗菌作用与海藻藻藻藻酸肠道肠杆菌肠杆菌肠杆菌分离的抗菌作用。

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摘要

This research study is about the antibacterial potential of protein and its hydrolysates isolated from bacteria Enterobacter hormaechei associated with marine algae. The protein was isolated using a fractionation method with ammonium sulphate. The fractions were pre-purified through dialysis with buffer Tris HCl. Protein hydrolysates were obtained through hydrolysis using the pepsin enzyme. Antibacterial activity was conducted using Escherichia coli and Staphylococcus aureus. The inhibition zone was observed after incubation for 24 h, 48 h, and 72 h at 37 °C. Results show that F1 fraction of protein hydrolysates had the highest activity against Escherichia coli and Staphylococcus aureus at 72 h with inhibition zones of 16.30±0.03 mm and 16.70±0.03 mm, respectively. Protein hydrolysates had higher inhibitory activity than the protein fraction, so it provides an information base for further study on the antibacterial drugs from bacteria associated with marine algae.
机译：该研究研究是关于蛋白质的抗菌潜力及其与海藻的细菌肠道胃肠杆菌分离的抗菌潜力。使用用硫酸铵的分馏方法分离蛋白质。通过用缓冲Tris HCl预先纯化级分。通过使用胃蛋白酶酶水解获得蛋白质水解产物。使用大肠杆菌和金黄色葡萄球菌进行抗菌活性。在37℃温育24小时，48小时和72小时后观察到抑制区。结果表明，蛋白质水解产物的F1分数在72小时的抑制区分别具有16.30±0.03mm和16.70±0.03mm的抑制区的72小时的最高活性蛋白质水解酸盐和金黄色葡萄球菌。蛋白质水解产物具有比蛋白质分数更高的抑制活性，因此它提供了进一步研究与海藻相关的细菌的抗菌药物的信息群。

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