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首页> 外文期刊>Pakistan journal of botany >Genetic diversity of Symplocos paniculata of Hunan province revealed by inter-simple sequence repeat (ISSR)
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Genetic diversity of Symplocos paniculata of Hunan province revealed by inter-simple sequence repeat (ISSR)

机译：湖南省股票帕诺皮塔帕皮皮塔的遗传多样性（ISSR）透露

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摘要

The natural populations’ genetic diversity of Symplocos paniculata in Hunan province was evaluated by using Inter-simple sequence repeat (ISSR) markers. In all 122 loci were identified in 149 individual plants of six populations using nine selected ISSR primers (UBC815, UBC817, UBC824, UBC835, UBC845, UBC853, UBC862, UBC873 and UBC879) and 92.2% loci were polymorphic. Nei’s gene diversity ( H ) and shannon information index ( I) were 0.3264 and 0.4873, respectively. The coefficient of gene differentiation ( Gst ) was 0.5397. Gene flow among populations ( Nm ) was 0.4763. Analysis of molecular variance (AMOVA) demonstrated that the genetic differentiation among S. paniculata populations was relatively high ( p 0.001). The among-population component accounted for 51.1% of the total variation, while the within-population component accounted for 49.8%. Six populations were clustered into three groups by the unweighted pair-group method arithmetic average (UPGMA). The results showed that populations of Da Wei Shan A (DWSA), Da Wei Shan B (DWSB) and Xiang Xi Long Shan (XXLS) were in the first group, populations of Yue Yang Jun Shan (YYJS) and Yong Zhou Dao Xian (YZDX) were in another group, and Heng Yang Hen Shan (HYHS) population separated from all the populations in the dendrogram. The cluster results were mutually related to the geographical elevation, but not to geographical distribution.
机译：利用简单的序列重复（ISSR）标记评估湖南省Symplocos Paniculata的自然群体遗传多样性。在所有122个基因座中，在使用九个所选ISSR引物（UBC815，UBC817，UBC824，UBC835，UBC845，UBC853，UBC862，UBC873和UBC879）和92.2％基因座中的六个群体的单个植物中鉴定了149个群体的植物。 Nei的基因多样性（h）和香农信息指数（i）分别为0.3264和0.4873。基因分化系数（GST）为0.5397。种群（NM）之间的基因流量为0.4763。分子方差分析（AMOVA）证明了S.Paniculata群中的遗传分化相对较高（P <0.001）。人口中的组成部分占总变异的51.1％，而人口内部分量占49.8％。通过未加权对 - 组方法算术平均值（UPGMA）将六个种群聚集成三组。结果表明，大卫山（DWSA），大卫山（DWSB）和Xiang Xi Long Shan（XXLS）的人口在第一组，岳阳君山（YYJS）和永周道县的人口（ yzdx）在另一组，衡阳母鸡（Hyhs）人口与树木图中的所有群体分开。群集结果与地理仰角相互关联，但不是地理分布。

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