• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Ukrainian Biochemical Journal >Freezing influences, the exposure of IgG glycans in sera from multiple sclerosis patients
【24h】

Freezing influences, the exposure of IgG glycans in sera from multiple sclerosis patients

机译：冷冻影响，来自多发性硬化症患者血清中IgG聚糖的暴露

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

N-glycan residues attached to Asn297 of the immunoglobulin IgG molecule are responsible for changing its structural conformation and are used as markers of many inflammatory diseases. Freezing stabilizes protein structure, while recent solution NMR data showed greatly altered IgG glycan mobility at different temperatures. The aim of the current work was to investigate whether freezing sera samples from multiple sclerosis (MS) patients and normal healthy donors (NHD) influences exposure of IgG glycans. The developed lectin immunosorbent assay was used to evaluate exposure of native IgG glycans with fucose-binding AAL lectin and sialic acid-binding SNA lectin. Sera samples were divided and either immediately frozen at -20 °C or stored at 4 °C. Lectin exposure was compared between 5 MS patient groups ( n = 75) vs NHD ( n = 23) and in paired samples with and without freezing. A significant increase in the exposure of fucose residues on IgG glycans in MS patients, compared to NHD, was observed. This increase was only observed if sera were frozen before analysis. The exposure of sialic acid was decreased in MS vs NHD samples after freezing sera samples. The exposure of core fucose residues and terminal sialic residues differed significantly in paired sera samples after freezing. Combined parameters of fucose and sialic acid exposure on native IgG glycans using frozen sera samples serve as a discriminative marker between MS and NHD. For AAL exposure, the discrimination of MS was characterized by AUROC of 0.906, sensitivity of 76.7% and specificity of 59.0% ( P 0.0001).
机译：附着于免疫球蛋白IgG分子的ASN297的N-聚糖残基是负责改变其结构构象的原因，用作许多炎症疾病的标志物。冷冻稳定蛋白质结构，而最近的溶液NMR数据显示在不同温度下大大改变了IgG聚糖迁移率。目前工作的目的是调查冻结来自多发性硬化症（MS）患者的血清样本和正常健康供体（NHD）影响IgG聚糖的暴露。研发的凝集素免疫吸附测定用于评估天然IgG聚糖与岩藻糖结合AAL凝集素和唾液酸结合SNA凝集素的暴露。将血清样品分开，并立即在-20℃下冷冻或在4℃下储存。在5ms患者基团（n = 75）与NHD（n = 23）和具有和不冷冻的配对样品之间进行比较凝集素。观察到与NHD相比，MS患者IgG聚糖对岩藻糖残基暴露的显着增加。只有在分析前血清被冷冻时才会观察到这种增加。在冷冻血清样品后，在MS VS NHD样品中，唾液酸的曝光降低。冻结后，核心岩藻糖残基和末端唾液酸残留物的曝光显着不同。使用冷冻血清样品对天然IgG聚糖对天然IgG聚糖的组合参数用作MS和NHD之间的鉴别标记。对于AAL暴露，MS的辨别表征为0.906的AUROC，敏感性为76.7％，特异性为59.0％（P <0.0001）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号