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首页> 外文期刊>Journal of molecular recognition: JMR >Substrate specificity of IgGs with peroxidase and oxidoreductase activities from sera of patients with systemic lupus erythematosus and multiple sclerosis
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Substrate specificity of IgGs with peroxidase and oxidoreductase activities from sera of patients with systemic lupus erythematosus and multiple sclerosis

机译：来自患有全身狼疮红斑狼疮和多发性硬化症患者血清的过氧化物酶和氧化还原酶活性的IgG的底物特异性

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Abstract The analysis of IgGs to protect humans from oxidative stress through oxidation of harmful compounds was carried out. We have compared here for the first time peroxidase (in the presence of H 2 O 2 ) and oxidoreductase (in the absence of H 2 O 2 ) activities of IgGs from sera of healthy humans and patients with systemic lupus erythematosus (SLE) and multiple sclerosis (MS). In addition, substrate specificity of SLE and MS IgG preparations in the oxidation of different compounds was analyzed: 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), 3,3′‐diaminobenzidine (DAB), homovanillic acid (HVA), o‐phenylenediamine (OPD), α‐naphthol, 3‐amino‐9‐ethylcarbazole (AEC), p ‐hydroquinone (pHQ), and adrenaline. IgGs of healthy humans and SLE and MS patients oxidized DAB, ABTS, and OPD due to their peroxidase and oxidoreductase activities, while other compounds were substrates of IgGs only in the presence of H 2 O 2 : adrenaline was not oxidized by both activities of IgGs. The average SLE IgGs peroxidase activity increased statistically significant in comparison with abzymes from healthy humans in the order (‐fold): OPD (1.2)?? DAB (1.7)??α‐naphtol (2.2)?≤?AEC (2.4)??ABTS (4.5)??5‐ASA (10.6), while with oxidoreductase activity: OPD (1.8)?≤?DAB (2.1‐fold)??ABTS (5.0). Only HVA was oxidized by IgGs with peroxidase activity of healthy donors faster than by SLE (1.3‐fold) and MS abzymes (2.4‐fold). In the oxidation of several substrates, only three IgGs of MS patients were used. The data speak of a tendency to increase the peroxidase and oxidoreductase activities of MS IgGs in comparison with healthy donors, but to a lesser extent: OPD (1.1 to 1.2‐fold)?≤?ABTS (1.2 to 1.8‐fold). It was shown that development of SLE and MS leads to increase in peroxidase and oxidoreductase activities of IgGs toward most of classical substrates. Thus, abzymes can serve as an additional factor of reactive oxygen species detoxification protecting of patients with SLE and MS from some harmful compounds somewhat better than healthy peoples.
机译：摘要进行了通过氧化有害化合物免受氧化应激保护人类的分析。我们已经在此进行了比较了第一次过氧化物酶（在H 2 O 2的存在下）和氧化还原酶（在没有H 2 O 2的情况下）来自健康人类的血清的IgGs和Systemic Lupus红斑狼疮（SLE）和多个硬化症（MS）。此外，分析了SLE和MS IgG制剂在不同化合物的氧化中的底物特异性：2,2'-唑苯基 - 双（3​​-乙基苯并噻唑啉-6-磺酸）（ABTS），3,3'-二氨基苯胺（DAB ），同源甲酸（HVA），O-苯二胺（OPD），α-萘酚，3-氨基-9-乙基咔唑（AEC），P-羟基喹啉（PHQ）和肾上腺素。由于其过氧化物酶和氧化还原酶活动，健康人和SLE和MS患者的IgGS氧化DAB，ABTS和OPD，而其他化合物仅在H 2 O 2的存在下仅在H 2 O 2的存在下进行亚苯甲酸酯。与来自秩序中的健康人类的戒烟（ - 折叠）：OPD（1.2）α，平均SLE IgGs过氧化物酶活性增加了统计学上显着的显着性dab（1.7）？α-α-萘醇（2.2）？≤αα≤αα≤αα-α≤α-······（4.5）（10.6），同时用氧化还原酶活性：OPD（1.8）？ ≤αβα（2.1倍）？α

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