...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Toxins >Role of Shiga Toxins in Cytotoxicity and Immunomodulatory Effects of Escherichia coli O157:H7 during Host-Bacterial Interactions in vitro
【24h】

Role of Shiga Toxins in Cytotoxicity and Immunomodulatory Effects of Escherichia coli O157:H7 during Host-Bacterial Interactions in vitro

机译：Shiga毒素在体外宿主细菌相互作用期间大肠杆菌O157：H7的体育毒性和免疫调节作用的作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Enterohemorrhagic Escherichia coli (EHEC) strains are food-borne pathogens that can cause different clinical conditions. Shiga toxin 2a and/or 2c (Stx2)-producing E. coli O157:H7 is the serotype most frequently associated with severe human disease. In this work we analyzed the hypothesis that host cells participate in Stx2 production, cell damage, and inflammation during EHEC infection. With this aim, macrophage-differentiated THP-1 cells and the intestinal epithelial cell line HCT-8 were incubated with E. coli O157:H7. A time course analysis of cellular and bacterial survival, Stx2 production, stx 2 transcription, and cytokine secretion were analyzed in both human cell lines. We demonstrated that macrophages are able to internalize and kill EHEC. Simultaneously, Stx2 produced by internalized bacteria played a major role in macrophage death. In contrast, HCT-8 cells were completely resistant to EHEC infection. Besides, macrophages and HCT-8 infected cells produce IL-1β and IL-8 inflammatory cytokines, respectively. At the same time, bacterial stx 2 -specific transcripts were detected only in macrophages after EHEC infection. The interplay between bacteria and host cells led to Stx production, triggering of inflammatory response and cell damage, all of which could contribute to a severe outcome after EHEC infections.
机译：Enterohemorrhagic大肠杆菌（EHEC）菌株是食品传播的病原体，可导致不同的临床病症。 Shiga毒素2a和/或2c（stx2） - 发挥大肠杆菌O157：H7是最常与严重人类疾病相关的血清型。在这项工作中，我们分析了宿主细胞在EHEC感染期间参与STX2生产，细胞损伤和炎症的假设。通过这种目的，将巨噬细胞分化的THP-1细胞和肠上皮细胞系HCT-8与大肠杆菌O157：H7一起温育。在两种人体细胞系中分析了细胞和细菌存活，STX2生产，STX 2转录和细胞因子分泌的时间过程分析。我们证明巨噬细胞能够内化和杀死EHEC。同时，内化细菌产生的STX2在巨噬细胞死亡中发挥了重要作用。相比之下，HCT-8细胞完全抵抗EHEC感染。此外，巨噬细胞和HCT-8感染的细胞分别产生IL-1β和IL-8炎性细胞因子。同时，在EHEC感染后，仅在巨噬细胞中检测细菌STX 2的特异性转录物。细菌和宿主细胞之间的相互作用导致STX生产，触发炎症反应和细胞损伤，所有这些都可能导致EHEC感染后的严重结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号