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首页> 外文期刊>Turkish Journal of Field Crops >Assessing genetic diversity of some potato (Solanum tuberosum L.) genotypes grown in Turkey using the AFLP marker technique
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Assessing genetic diversity of some potato (Solanum tuberosum L.) genotypes grown in Turkey using the AFLP marker technique

机译：使用AFLP标记技术评估在土耳其生长的土豆（Solanum Tuberosum L.）基因型的遗传多样性

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In the present study, the level of polymorphism and the genetic relationships among 26 potato genotypes were studiedby means of molecular markers using the amplified fragment length polymorphism (AFLP) technique. DNA wasextracted from fresh leaves of the seedlings. Selective amplification products revealed a total of 191 polymorphicbands ranging from 8 to 45 for each combination. Scoring results were used to generate a tree in JMP software. The26 samples formed six clades with varying number of members between one and eleven. Genetic distances amonggenotypes were calculated according to Jaccard’s formula, in Phylip 3.0 software. According to the results of geneticdistance, dendrogram showed that genotypes 6/7-4 and 6/7-2 were the closest genotypes with a genetic distance of 0.13.On the other hand, genotypes Posof-10 and Marabel were the most distinct from each other with a genetic distancevalue of 0.55. The AFLP marker results showed a great consistency along with their pedigrees indicating the AFLPtechnique as a useful tool in the calculation of genetic distance of the potato genotypes.
机译：在本研究中，使用扩增的片段长度多态性（AFLP）技术，研究了26种马铃薯基因型中的多态性和遗传关系。 DNA从幼苗的新鲜叶子中脱胶。选择性扩增产物揭示了总共191种多态性，每个组合的8至45次。评分结果用于在JMP软件中生成一棵树。 The26样品形成六个片状，其中一个和11之间的变化数量不同。根据Jaccard的公式，在Phylip 3.0软件中计算遗传距离。根据遗传等级的结果，树木图显示基因型6 / 7-4和6/7-2是最接近的基因型，遗传距离为0.13.另一方面，基因型POSOF-10和马拉布尔是最截然的其他遗传距离为0.55。 AFLP标记结果显示出良好的一致性以及它们的章程，将AFLPTECHNIQUE作为一种有用的工具，以计算马铃薯基因型的遗传距离。

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