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首页> 外文期刊>Potato Research >Genetic Diversity of Chinese and CIP Potato (Solanum tuberosum L.) Germplasm Assessed by Amplified Fragment Length Polymorphism (AFLP) Markers
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Genetic Diversity of Chinese and CIP Potato (Solanum tuberosum L.) Germplasm Assessed by Amplified Fragment Length Polymorphism (AFLP) Markers

机译：利用扩增片段长度多态性（AFLP）标记评估中国和CIP马铃薯（Solanum tuberosum L.）种质的遗传多样性

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摘要

The genetic diversity of 123 potato clones, consisting of 103 clonal accessions from the International Potato Center (CIP) and 20 Chinese cultivars, was assessed using amplified fragment length polymorphism (AFLP) markers. Ten polymorphic primer combinations were selected to analyse and compare the diversity of the two sources of clones. A total of 521 reproducible bands were amplified, of which 488 were polymorphic. These AFLP markers were analysed to estimate the genetic distance (GD) and genetic similarity (GS) coefficient of all clones. The GD between pairs of clones ranged from 0.03 to 0.48. Nei’s gene diversity index was between 0.236 and 0.387, with an average of 0.330. The index of Shannon’s information varied from 0.361 to 0.567 with an average of 0.498. A high degree of polymorphism was observed with an average of 93.6% loci found to be polymorphic. Cluster analysis showed that the CIP accessions were grouped together at the GS 0.59 clustering line, whereas most Chinese cultivars grouped at the GS 0.82 clustering line. The diversity in CIP potato resources was found to be higher than that of the Chinese cultivars, indicating that the genetic base of Chinese potato cultivars is narrow and may benefit from broadening.
机译：使用扩增片段长度多态性（AFLP）标记评估了123个马铃薯无性系的遗传多样性，其中包括来自国际马铃薯中心（CIP）的103个克隆种和20个中国品种。选择了十个多态性引物组合以分析和比较两种克隆来源的多样性。总共扩增了521条可再现的条带，其中488条是多态的。分析这些AFLP标记以估计所有克隆的遗传距离（GD）和遗传相似性（GS）系数。克隆对之间的GD在0.03至0.48之间。 Nei的基因多样性指数在0.236和0.387之间，平均为0.330。香农信息的指数从0.361到0.567，平均为0.498。观察到高度的多态性，平均93.6％的基因座被发现是多态性的。聚类分析表明，CIP品种在GS 0.59聚类线上分组在一起，而大多数中国品种在GS 0.82聚类线上分组。发现CIP马铃薯资源的多样性高于中国品种，表明中国马铃薯品种的遗传基础狭窄，可能会受益于拓宽。

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  • 来源
    《Potato Research》 |2013年第3期|167-178|共12页
  • 作者

    Fang Wang; Fang-di Li; Jian Wang;

  • 作者单位

    Institute of Biotechnology of Qinghai Academy of Agriculture and Forestry">(1);

    The Qinghai-Tibet Plateau Biotechnology Key Lab of Ministry of Education">(2);

    Germplasm Innovation and Utilization of Plateau Crop Key Laboratory of Qinghai Province">(4);

    Tianshui Institute of Agricultural Sciences">(3);

    Institute of Biotechnology of Qinghai Academy of Agriculture and Forestry">(1);

    The Qinghai-Tibet Plateau Biotechnology Key Lab of Ministry of Education">(2);

    Germplasm Innovation and Utilization of Plateau Crop Key Laboratory of Qinghai Province">(4);

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    AFLP; Genetic diversity; Germplasm; Potato;

    机译：AFLP;遗传多样性;种质;土豆;

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