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首页> 外文期刊>Journal of genetics >Evaluation of Drosophila chromosomal segments proposed by means of simulations of possessing hybrid sterility genes from reproductive isolation
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Evaluation of Drosophila chromosomal segments proposed by means of simulations of possessing hybrid sterility genes from reproductive isolation

机译：通过模拟从生殖隔离的杂种不良基因的模拟提出的果蝇染色体段的评价

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In heterozygote state, we interogressed three chromosomal segments of Drosophila koepferae in D. buzzatii. The effect of each introgression was evaluated in the fertility of the segmental males, quantifying the amount of offspring produced. Through specific crosses method, we generated Drosophila segmental isolines carrying specific chromosomal introgression segments. The introgressions were monitored cytogenetically by the method of molecular markers of chromosomal asynapsis. The statistical analysis showed that none of the three segments evaluated, introgressed individually or in pairs, as well as cis or trans, do not produce sterility in the segmental males, as determined by the normal productions of offspring. Additional introgressions using other larger segments show that when the introgressions reach a minimum size of 31.15%, they produce sterility. It is concluded that the hybrid sterility genes present in the three segments evaluated did not act in strong epistasis, but show a pattern of gradual additive behaviour by requiring a minimum threshold size to produce sterility. Finally, we also isolated the smallest introgressing segment that has been reported for these species (2.19%), and for the first time we have managed to place it in homozygous state (data not shown), so we are now in the process of evaluating the ability to these segments in homozygous state.
机译：在杂合子状态下，我们在D.Buzzatii中交流了三种果蝇Koepferae的三种染色体片段。每次血栓增长的效果在节段性男性的生育率中评估，量化所产生的后代的量。通过特定的交叉方法，我们产生了携带特异性染色体血栓段的果蝇节段性分离型。通过染色体Anynapsis的分子标记的方法来监测细胞突出菌。统计分析表明，单独或成对的三个段中没有出来，以及CIS或反式，在节段性雄性中不产生不育，如后代正常制作所确定的。使用其他较大段的额外迟滞表明，当速增渗达到31.15％的最小尺寸时，它们会产生无菌性。结论是，评估的三个区段中存在的杂化不育基因并未在强大的外观中起作用，但通过需要最小阈值尺寸以产生无菌的逐渐添加性行为的模式。最后，我们还孤立了这些物种（2.19％）的最小斜突部分（2.19％），我们第一次设法将其放在纯合状态（未显示的数据），所以我们现在正在评估的过程中对纯合状态下这些段的能力。

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