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首页> 外文期刊>Journal of International Medical Research >Bioinformatics analysis of different candidate genes involved in hepatocellular carcinoma induced by HepG2 cells or tumor cells of patients
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Bioinformatics analysis of different candidate genes involved in hepatocellular carcinoma induced by HepG2 cells or tumor cells of patients

机译：患者肝细胞癌诱导的不同候选基因的生物信息分析

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Objective Hepatocellular carcinoma (HCC) is a common cancer with a high mortality rate; the molecular mechanism involved in HCC remain unclear. We aimed to provide insight into HCC induced with HepG2 cells and identify genes and pathways associated with HCC, as well as potential therapeutic targets. Methods Dataset GSE72581 was downloaded from the Gene Expression Omnibus, including samples from mice injected in liver parenchyma with HepG2 cells, and from mice injected with cells from patient tumor explants. Differentially expressed genes (DEGs) between the two groups of mice were analyzed. Then, gene ontology and Kyoto Encyclopedia of Gene and Genomes pathway enrichment analyses were performed. The MCODE plug-in in Cytoscape was applied to create a protein–protein interaction (PPI) network of DEGs. Results We identified 1,405 DEGs (479 upregulated and 926 downregulated genes), which were enriched in complement and coagulation cascades, peroxisome proliferator-activated receptor signaling pathway, and extracellular matrix–receptor interaction. The top 4 modules and top 20 hub genes were identified from the PPI network, and associations with overall survival were determined using Kaplan–Meier analysis. Conclusion This preclinical study provided data on molecular targets in HCC that could be useful in the clinical treatment of HCC.
机译：目标肝细胞癌（HCC）是一种常见的癌症，具有高死亡率;涉及HCC的分子机制仍不清楚。我们旨在提供对HEP2细胞诱导的HCC的洞察，并鉴定与HCC相关的基因和途径以及潜在的治疗目标。方法从基因表达Omnibus下载DataSet GSE72581，包括来自肝脏实质的小鼠的样品用HepG2细胞，以及从患者肿瘤外植体注射细胞的小鼠。分析了两组小鼠之间的差异表达基因（DEGS）。然后，进行基因本体和京都基因的细胞科和基因组途径富集分析。施用Cytoscape中的MCODE插入以产生蛋白质 - 蛋白质相互作用（PPI）网络的DEG。结果我们鉴定了1,405℃（479个上调和926个下调基因），其富含补体和凝固级联，过氧化物体增殖物激活的受体信号通路和细胞外基质受体相互作用。从PPI网络中鉴定了前4个模块和前20个枢纽基因，并使用Kaplan-Meier分析确定与总存活的关联。结论该临床前研究提供了关于HCC中的分子靶标的数据，其可用于HCC的临床治疗。

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