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首页> 外文期刊>Journal of King Saud University >Molecular characterization of goats from Saudi Arabia using microsatellite markers
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Molecular characterization of goats from Saudi Arabia using microsatellite markers

机译：使用微卫星标记的沙特阿拉伯山羊的分子特征

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摘要

This study is undertaken for genetic characterization of local Saudi goat populations for maintaining genetic variability of livestock population. Genomic DNA was extracted from three goat populations namely Ardi, Hollandi and Shami and subjected for genotyping using eighteen recommended microsatellite markers. The average values obtained for number of private alleles, effective number of alleles and different alleles 2.071, 5.343 and 9.389, respectively. The expected and observed heterozygosity values were 0.757 and 0.913, respectively. F- values were 0.081, ?0.115, ?0.18 and Shannon's Information Index was found to be 1.751. All loci were highly differed from Hardy-Weinburg Equilibrium and an average migrant’s value was 4.302. The maximum value was obtained between Hollandi and Ardi (8.474) and the value was extremely low between Shami and Ardi (2.518). Pairwise population of Fst and Nei's Genetic Distance showed very close relationship between Hollandi and Ardi goat population than between this population with the Shami. STRUCTURE software revealed 3 clusters at K?=?3 in goat population. The present findings revealed genetic variations in Hollandi and Ardi populations. Molecular characterization of Saudi goats is important for maintaining and improving genetic resources.
机译：本研究是为了维持牲畜群的遗传变异的地方沙特山羊群的遗传表征。基因组DNA从三个山羊种群中提取，即Ardi，Hollandi和Shami，并使用18个推荐的微卫星标记进行基因分型。为私人等位基因数，有效数量的等位基因数和不同等位基因2.071,5.343和9.389获得的平均值。预期和观察到的杂合子值分别为0.757和0.913。 F值为0.081，？0.115，？0.18和香农信息指数被发现为1.751。所有基因座都与Hardy-Weinburg均衡高度不同，平均移民价值为4.302。在Hollandi和Ardi之间获得的最大值（8.474），Shami和Ardi（2.518）之间的值极低。 FST和Nei的遗传距离的成对群体在荷兰迪和Ardi山羊人口之间具有比与Shami的人口之间非常密切的关系。结构软件在山羊人口中揭示了3个群集=？3。本研究结果揭示了荷兰迪和艾迪群体的遗传变异。沙特山羊的分子表征对于维持和改善遗传资源很重要。

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