首页> 外文期刊>Journal of King Saud University >In vitro evaluation of antifungal activity of some agricultural fungicides against two saprolegnoid fungi infecting cultured fish
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In vitro evaluation of antifungal activity of some agricultural fungicides against two saprolegnoid fungi infecting cultured fish

机译:两种农业杀菌剂对两种Saprongnoid真菌感染培养鱼的体外评价

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BackgroundSaprolegniasis often cause a significant economic losses to fish hatchery and intensive fish industry. The treatment of saprolegnoid diseases with malachite green seems to have harmful effect and is considered as a mutagenic and carcinogenic substance. The teratogenic and carcinogenic potential of malachite green increased the necessity to find an effective alternative treatment to control the disease.MethodsIn the present study, seven fungicides used in agriculture were investigated to controlSaprolegnia parasiticaandS. diclina in vitro. The antifungal activity of each fungicide was compared with that of malachite green as reference fungicide using food poisoning technique. Fish toxicity of the effective fungicides was performed to detect the most applicable fungicides in fish aquarium.ResultsFour of seven fungicides were found to be effective against the two saprolegnoid fungi. Azoxystrobin and metalaxyl were the most effective fungicides inhibited fungal growth of the two saprolegnoid fungi completely at concentration of 200?ppm and 250?ppm respectively while cyazofamid and etridiazole were moderately effective. Acute toxicity assay of the two effective fungicides showed metalaxyl was low toxic to fish with LC50of 360?ppm while azoxystrobin was 225?ppm. Hence, it was confirmed that 200 and 250?ppm of azoxystrobin and metalaxyl which completely inhibited mycelial growth of the saprolegniod fungi could be safely used for saprolegniasis control.ConclusionThese fungicides which proved to be effective and fishery safer may be applicable as a aquatic fungicides avoiding teratogenic and carcinogenic risk of malachite green.
机译:BackgroundSapraLegniasis经常导致鱼类孵化和密集的鱼类行业产生重大的经济损失。用孔雀石绿色的Saprongnoid疾病的治疗似乎具有有害影响,被认为是诱变和致癌物质。孔雀石绿的致畸和致癌潜力增加了寻找有效替代治疗以控制疾病的必要性。目前的研究,农业中使用的7种杀菌剂对ControlSaprolegnia寄生虫进行了研究。 Diclina体外。使用食物中毒技术将每种杀真菌剂的抗真菌活性与孔雀石绿色的抗真菌活性进行比较。进行了有效杀菌剂的鱼类毒性,以检测鱼类水族馆中最适用的杀真菌剂。发现七种杀菌剂的结果是有效的,对两种SaProngnoid真菌有效。偶氮氧菊酯和金属酰基是最有效的杀菌剂,抑制了两个Saprongnoid真菌的真菌生长,分别以200〜ppm和250μlβppm的浓度完全浓缩,而Cyazofamid和etridiazole适度有效。两种有效的杀菌剂的急性毒性测定显示金属酰基含有LC50.PPM的毒性毒性低,氮氧蛋白为225μm≤PPM。因此,证实了200和250μlαppm的偶氮毒素和金属酰基,其完全抑制sapregniod真菌的菌丝体生长,可以安全地用于sapregniasis对照。证明是有效的杀菌剂,渔业更安全可用作避免水生真菌剂畸形和致癌术术的致致致致癌危险。

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